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PMID: 2124704 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 24 ·1990-12-00 ·Pages 9853-7

Shinjo K, Koland JG, Hart MJ, Narasimhan V, Johnson DI, Evans T, Cerione RA

Abstract

We have isolated cDNA clones from a human placental library that code for a low molecular weight GTP-binding protein originally designated Gp (also called G25K). This identification is based on comparisons with the available peptide sequences for the purified human Gp protein and the use of two highly specific anti-peptide antibodies. The predicted amino acid sequence of the protein is very similar to those of various members of the ras superfamily of low molecular weight GTP-binding proteins, including the N-, Ki-, and Ha-ras proteins (30-35% identical), the rho proteins (approximately 50% identical), and the rac proteins (approximately 70% identical). The highest degree of sequence identity (80%) is found with the Saccharomyces cerevisiae cell-division-cycle protein CDC42. The human placental gene, which we designate CDC42Hs, complements the cdc42-1 mutation in S. cerevisiae, which suggests that this GTP-binding protein is the human homolog of the yeast protein.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Cycle Cell Division Cloning, Molecular Escherichia coli/genetics Fungal Proteins/genetics GTP-Binding Proteins/genetics,metabolism Genes Genetic Complementation Test Guanosine 5'-O-(3-Thiotriphosphate)/metabolism Humans Kinetics Molecular Sequence Data Mutation Placenta/metabolism Pregnancy Proteins/genetics Restriction Mapping Saccharomyces cerevisiae/cytology,genetics Sequence Homology, Nucleic Acid cdc42 GTP-Binding Protein cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
Chemicals
Fungal Proteins Pregnancy Proteins Guanosine 5'-O-(3-Thiotriphosphate) GTP-Binding Proteins cdc42 GTP-Binding Protein cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Shinjo K
Department of Pharmacology, Cornell University, Ithaca, NY 14850.
Koland J G
Hart M J
Narasimhan V
Johnson D I
Evans T
Cerione R A
References (26)
26 references, click to expand
  1. A novel small molecular weight GTP-binding protein with the same putative effector domain as the ras proteins in bovine brain membranes. Purification, determination of primary structure, and characterization.
    J Biol Chem. 1988 Dec 15;263(35):18965-71 PMID: 3143720
  2. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
    Gene. 1988 Jul 15;67(1):31-40 PMID: 3047011
  3. Characterization of G25K, a GTP-binding protein containing a novel putative nucleotide binding domain.
    Biochem Biophys Res Commun. 1989 Apr 14;160(1):25-32 PMID: 2496687
  4. rac, a novel ras-related family of proteins that are botulinum toxin substrates.
    J Biol Chem. 1989 Oct 5;264(28):16378-82 PMID: 2674130
  5. Identification of the ral and rac1 gene products, low molecular mass GTP-binding proteins from human platelets.
    J Biol Chem. 1989 Oct 5;264(28):16383-9 PMID: 2550440
  6. Expression of epidermal growth factor receptor sequences as E. coli fusion proteins: applications in the study of tyrosine kinase function.
    Biochem Biophys Res Commun. 1990 Jan 15;166(1):90-100 PMID: 2154210
  7. Molecular switch for signal transduction: structural differences between active and inactive forms of protooncogenic ras proteins.
    Science. 1990 Feb 23;247(4945):939-45 PMID: 2406906
  8. The identification and characterization of an epidermal growth factor-stimulated phosphorylation of a specific low molecular weight GTP-binding protein in a reconstituted phospholipid vesicle system.
    J Biol Chem. 1990 Apr 15;265(11):5990-6001 PMID: 2156836
  9. CDC42 and CDC43, two additional genes involved in budding and the establishment of cell polarity in the yeast Saccharomyces cerevisiae.
    J Cell Biol. 1990 Jul;111(1):131-42 PMID: 2195038
  10. Molecular characterization of CDC42, a Saccharomyces cerevisiae gene involved in the development of cell polarity.
    J Cell Biol. 1990 Jul;111(1):143-52 PMID: 2164028
  11. The cellular functions of small GTP-binding proteins.
    Science. 1990 Aug 10;249(4969):635-40 PMID: 2116664
  12. Molecular cloning and expression of a G25K cDNA, the human homolog of the yeast cell cycle gene CDC42.
    Mol Cell Biol. 1990 Nov;10(11):5977-82 PMID: 2122236
  13. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  14. A yeast gene encoding a protein homologous to the human c-has/bas proto-oncogene product.
    Nature. 1983 Dec 15-21;306(5944):704-7 PMID: 6318115
  15. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  16. A novel ras-related gene family.
    Cell. 1985 May;41(1):31-40 PMID: 3888408
  17. Purification of the major GTP-binding proteins from human placental membranes.
    J Biol Chem. 1986 May 25;261(15):7052-9 PMID: 3084494
  18. A ras-like protein is required for a post-Golgi event in yeast secretion.
    Cell. 1987 May 22;49(4):527-38 PMID: 3552249
  19. ras genes.
    Annu Rev Biochem. 1987;56:779-827 PMID: 3304147
  20. Vectors for selective expression of cloned DNAs by T7 RNA polymerase.
    Gene. 1987;56(1):125-35 PMID: 3315856
  21. Four additional members of the ras gene superfamily isolated by an oligonucleotide strategy: molecular cloning of YPT-related cDNAs from a rat brain library.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8210-4 PMID: 3317403
  22. Identification and purification from bovine brain of a guanine-nucleotide-binding protein distinct from Gs, Gi and Go.
    Biochem J. 1987 Sep 1;246(2):431-9 PMID: 3120696
  23. Purification and characterization of a GTP-binding protein with a molecular weight of 20,000 in bovine brain membranes. Identification as the rho gene product.
    J Biol Chem. 1988 Jul 15;263(20):9926-32 PMID: 3133371
  24. Sequences of the bovine and yeast ADP-ribosylation factor and comparison to other GTP-binding proteins.
    Proc Natl Acad Sci U S A. 1988 Jul;85(13):4620-4 PMID: 3133654
  25. Human cDNAs rap1 and rap2 homologous to the Drosophila gene Dras3 encode proteins closely related to ras in the 'effector' region.
    Oncogene. 1988 Aug;3(2):201-4 PMID: 3045729
  26. A ras-related gene with transformation suppressor activity.
    Cell. 1989 Jan 13;56(1):77-84 PMID: 2642744
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.24.9853
Published
1990-12-00
Pages
9853-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC55272
Subset
IM
Grants
NEI NIH HHS · EY06429 · United States
NIGMS NIH HHS · GM40654 · United States
Databases
GENBANK
M57298
External Links
PubMed source DOI: 10.1073/pnas.87.24.9853 PMC Full Text View journal details
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