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PMID: 2124708 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mutation in a heterochromatin-specific chromosomal protein is associated with suppression of position-effect variegation in Drosophila melanogaster.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 24 ·1990-12-00 ·Pages 9923-7

Eissenberg JC, James TC, Foster-Hartnett DM, Hartnett T, Ngan V, Elgin SC

Abstract

We report here that a point mutation in the gene which encodes the heterochromatin-specific nonhistone chromosomal protein HP-1 in Drosophila melanogaster is associated with dominant suppression of position-effect variegation. The mutation, a G-to-A transition at the first nucleotide of the last intron, causes missplicing of the HP-1 mRNA. This suggests that heterochromatin-specific proteins play a central role in the gene suppression associated with heterochromatic position effects.

MeSH Terms
Alleles Amino Acid Sequence Animals Base Sequence Blotting, Southern Chromobox Protein Homolog 5 Chromosomal Proteins, Non-Histone/genetics Cloning, Molecular DNA/genetics,isolation & purification Drosophila melanogaster/genetics Gene Library Heterochromatin/metabolism Molecular Sequence Data Mutation Oligonucleotide Probes Polymerase Chain Reaction Restriction Mapping Suppression, Genetic
Chemicals
Chromosomal Proteins, Non-Histone Heterochromatin Oligonucleotide Probes Chromobox Protein Homolog 5 DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Eissenberg J C
E. A. Doisy Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, MO 63104.
James T C
Foster-Hartnett D M
Hartnett T
Ngan V
Elgin S C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.24.9923
Published
1990-12-00
Pages
9923-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC55286
Subset
IM
Grants
NIGMS NIH HHS · GM09214 · United States
NIGMS NIH HHS · GM40732 · United States
NICHD NIH HHS · HD23844 · United States
Databases
GENBANK
M33749, M57574
External Links
PubMed source DOI: 10.1073/pnas.87.24.9923 PMC Full Text View journal details
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