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PMID: 2126058 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

High efficiency introduction of plasmid DNA into glycine treated Enterococcus faecalis by electroporation.

Molecular & general genetics : MGG ·Vol. 224 ·No. 1 ·1990-10-00 ·Pages 152-4

Cruz-Rodz AL, Gilmore MS

Abstract

A highly efficient electroporation system for Enterococcus faecalis was developed by systematically optimizing different parameters. One parameter found to be particularly critical for electroporation was cultivation of E. faecalis in medium containing a high glycine concentration, prior to electroporation. Osmotic stabilization of cells with 0.5 M sucrose was also found to be critical during glycine treatment. 10(6) transformants per microgram of plasmid DNA were consistently obtained within 48 h. Electrocompetent preparations of E.-faecalis could be stored at - 70 degrees C without loss of competence.

MeSH Terms
DNA, Bacterial/metabolism Enterococcus faecalis/genetics Glycine/pharmacology Osmolar Concentration Plasmids Transformation, Bacterial
Chemicals
DNA, Bacterial Glycine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cruz-Rodz A L
Department of Microbiology and Immunology, University of Oklahoma, Oklahoma City 73190.
Gilmore M S
References (19)
19 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00259462
Published
1990-10-00
Pages
152-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NEI NIH HHS · EY08289 · United States
External Links
PubMed source DOI: 10.1007/BF00259462
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