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PMID: 21264258 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Role of microRNA-26b in glioma development and its mediated regulation on EphA2.

PloS one ·Vol. 6 ·No. 1 ·2011-01-14 ·Pages e16264

Wu N, Zhao X, Liu M, Liu H, Yao W, Zhang Y, Cao S, Lin X

Abstract

MicroRNAs (miRNAs) are short, non-coding RNAs that regulate the expression of multiple target genes. Deregulation of miRNAs is common in human tumorigenesis. Low level expression of miR-26b has been found in glioma cells. However, its underlying mechanism of action has not been determined. Real-time PCR was employed to measure the expression level of miR-26b in glioma patients and cells. The level of miR-26b was inversely correlated with the grade of glioma. Ectopic expression of miR-26b inhibited the proliferation, migration and invasion of human glioma cells. A binding site for miR-26b was identified in the 3'UTR of EphA2. Over-expression of miR-26b in glioma cells repressed the endogenous level of EphA2 protein. Vasculogenic mimicry (VM) experiments were performed to further confirm the effects of miR-26b on the regulation of EphA2, and the results showed that miR-26b inhibited the VM processes which regulated by EphA2. This study demonstrated that miR-26b may act as a tumor suppressor in glioma and it directly regulates EphA2 expression. EphA2 is a direct target of miR-26b, and the down-regulation of EphA2 mediated by miR-26b is dependent on the binding of miR-26b to a specific response element of microRNA in the 3'UTR region of EphA2 mRNA.

MeSH Terms
3' Untranslated Regions Binding Sites/genetics Gene Expression Regulation, Neoplastic Glioma/etiology,genetics,pathology Humans MicroRNAs/physiology RNA, Messenger/genetics Receptor, EphA2/genetics Response Elements/genetics Tumor Suppressor Proteins
Chemicals
3' Untranslated Regions MIRN26A microRNA, human MicroRNAs RNA, Messenger Tumor Suppressor Proteins Receptor, EphA2
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Wu Ning
Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
Zhao Xiangzhong
Liu Ming
Liu Haizhou
Yao Weicheng
Zhang Yuyan
Cao Shousong
Lin Xiukun
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0016264
Published
2011-01-14
Epub
2011-00-14
Pages
e16264
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3021542
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0016264 PMC Full Text View journal details
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