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PMID: 212686 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Effects of lithium and thallous ions on sodium pump activity in the guinea-pig heart and their relationship to the positive inotropic action.

Naunyn-Schmiedeberg's archives of pharmacology ·Vol. 304 ·No. 2 ·1978-09-00 ·Pages 167-73

Ku DD, Akera T, Olgaard MK, Brody TM

Abstract

It has recently been demonstrated that both Tl+ and Li+ produce concentration- and time-dependent positive inotropic effects in guinea-pig atrial preparations although Tl+ inhibits and Li+ stimulates isolated Na+,K+-ATPase in vitro. In order to elucidate the mechanism of the positive inotropic actions of these cations, the effects of Tl+ and Li+ on sodium pump activity were studied. Active 86Rb uptake in guinea-pig ventricular slices, an estimate of sodium pump activity, was highly sensitive to the inhibitory effect of the cardiac glycosides. Preincubation of slices with Tl+ caused a dose- and time-dependent inhibition of active 86Rb uptake. Similar concentration- and time-dependent inhibition of active 86Rb uptake was observed when Na+ in a Krebs-Henseleit solution was partially replaced with Li+. Lithium, however, stimulated a partially purified Na+,K+-ATPase in vitro. During heart slice incubation, Tl+ and Li+ accumulated in a time-dependent manner. This accumulation was not readily reversible when slices were transferred into Tl+- or Li+-free solutions. It appears that the inhibition of sodium pump activity is related to the positive inotropic action of these cations.

MeSH Terms
Animals Female Guinea Pigs In Vitro Techniques Lithium/pharmacology Male Myocardial Contraction/drug effects Myocardium/enzymology,metabolism Radioisotopes Rubidium Sodium/metabolism Sodium-Potassium-Exchanging ATPase/metabolism Thallium/pharmacology
Chemicals
Radioisotopes Lithium Sodium Thallium Sodium-Potassium-Exchanging ATPase Rubidium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ku D D
Akera T
Olgaard M K
Brody T M
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31 references, click to expand
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Article Info
Journal
Naunyn-Schmiedeberg's archives of pharmacology
Abbr.
Naunyn Schmiedebergs Arch Pharmacol
ISSN
0028-1298
DOI
10.1007/BF00495553
Published
1978-09-00
Pages
167-73
Language
English
Region
Germany
NLM ID
0326264
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00495553 View journal details
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