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PMID: 212753 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Another chromosomal assignment for a simian virus 40 integration site in human cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 9 ·1978-09-00 ·Pages 4460-4

Kucherlapati R, Hwang SP, Shimizu N, McDougall JK, Botchan MR

Abstract

Somatic cell hybrids derived from fusion of GM637, a human cell line transformed by simian virus 40, and mouse B82 cells were examined for simian virus 40 T antigen, V antigen, and viral DNA. All hybrid cell lines that contained viral DNA were T-antigen positive. Cells that did not have viral DNA were T-antigen negative. We determined that there is a single viral insertion in these hybrid cells. Correlation of T-antigen expression and viral DNA with the partial complements of the human genome retained in the hybrids shwed that the inserted viral genome is in human chromosome 8. The integrated viral DNA is stable; free viral DNA found in GM637 does not insert at other potential sites in the human genome.

MeSH Terms
Antigens, Viral/analysis Cell Line Chromosomes, Human, 6-12 and X DNA, Viral/analysis,metabolism Humans Hybrid Cells/microbiology Simian virus 40/genetics,immunology Virus Replication
Chemicals
Antigens, Viral DNA, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kucherlapati R
Hwang S P
Shimizu N
McDougall J K
Botchan M R
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.9.4460
Published
1978-09-00
Pages
4460-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336135
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.9.4460 PMC Full Text View journal details
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