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PMID: 21288872 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Role of elongator subunit Elp3 in Drosophila melanogaster larval development and immunity.

Genetics ·Vol. 187 ·No. 4 ·2011-04-00 ·Pages 1067-75

Walker J, Kwon SY, Badenhorst P, East P, McNeill H, Svejstrup JQ

Abstract

The Elongator complex has been implicated in several cellular processes, including gene expression and tRNA modification. We investigated the biological importance of the Elp3 gene in Drosophila melanogaster. Deletion of Elp3 results in larval lethality at the pupal stage. During early development, larval growth is dramatically impaired, with progression to the third instar delayed for ∼24 hr, and pupariation occurring only at day 14 after egg laying. Melanotic nodules appear after 4 days. Microarray analysis shows that stress response genes are induced and ecdysone-induced transcription factors are severely repressed in the mutant. Interestingly, the phenotypes of Elp3 flies are similar to those of flies lacking the domino gene, encoding a SWI/SNF-like ATP-dependent chromatin-remodeling enzyme. Indeed, the gene expression profiles of these mutants are also remarkably similar. Together, these data demonstrate that Drosophila Elp3 is essential for viability, normal development, and hematopoiesis and suggest a functional overlap with the chromatin remodeler Domino.

MeSH Terms
Animals Chromatin/genetics,metabolism Chromatin Assembly and Disassembly Drosophila Proteins/genetics Drosophila melanogaster/genetics,growth & development Ecdysone/physiology Gene Deletion Gene Expression Profiling Gene Expression Regulation, Developmental Genes, Lethal Histone Acetyltransferases/genetics,metabolism Larva/genetics,metabolism Linear Models Nerve Tissue Proteins/genetics,metabolism Oligonucleotide Array Sequence Analysis Pupa/metabolism Transcription Factors/genetics,metabolism
Chemicals
Chromatin Drosophila Proteins Nerve Tissue Proteins Transcription Factors Ecdysone ELP3 protein, Drosophila Histone Acetyltransferases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Walker Jane
Clare Hall Laboratories, Cancer Research UK London Research Institute, South Mimms EN6 3LD, United Kingdom. [email protected]
Kwon So Yeon
Badenhorst Paul
East Phil
McNeill Helen
Svejstrup Jesper Q
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
1943-2631
DOI
10.1534/genetics.110.123893
Email
[email protected]
Published
2011-04-00
Epub
2011-00-01
Pages
1067-75
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC3070516
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BB/D522470/1 · United Kingdom
Cancer Research UK · United Kingdom
External Links
PubMed source DOI: 10.1534/genetics.110.123893 PMC Full Text View journal details
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