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PMID: 21335376 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

12-oxo-phytodienoic acid accumulation during seed development represses seed germination in Arabidopsis.

The Plant cell ·Vol. 23 ·No. 2 ·2011-02-00 ·Pages 583-99

Dave A, Hernández ML, He Z, Andriotis VM, Vaistij FE, Larson TR, Graham IA

Abstract

Arabidopsis thaliana COMATOSE (CTS) encodes an ABC transporter involved in peroxisomal import of substrates for β-oxidation. Various cts alleles and mutants disrupted in steps of peroxisomal β-oxidation have previously been reported to exhibit a severe block on seed germination. Oxylipin analysis on cts, acyl CoA oxidase1 acyl CoA oxidase2 (acx1 acx2), and keto acyl thiolase2 dry seeds revealed that they contain elevated levels of 12-oxo-phytodienoic acid (OPDA), jasmonic acid (JA), and JA-Ile. Oxylipin and transcriptomic analysis showed that accumulation of these oxylipins occurs during late seed maturation in cts. Analysis of double mutants generated by crossing cts with mutants in the JA biosynthesis pathway indicate that OPDA, rather than JA or JA-Ile, contributes to the block on germination in cts seeds. We found that OPDA was more effective at inhibiting wild-type germination than was JA and that this effect was independent of CORONATINE INSENSITIVE1 but was synergistic with abscisic acid (ABA). Consistent with this, OPDA treatment increased ABA INSENSITIVE5 protein abundance in a manner that parallels the inhibitory effect of OPDA and OPDA+ABA on seed germination. These results demonstrate that OPDA acts along with ABA to regulate seed germination in Arabidopsis.

MeSH Terms
Abscisic Acid/metabolism Arabidopsis/drug effects,genetics,growth & development Cyclopentanes/metabolism Fatty Acids, Unsaturated/pharmacology Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Germination/drug effects Mutation Oxylipins/metabolism Seeds/drug effects,growth & development
Chemicals
Cyclopentanes Fatty Acids, Unsaturated Oxylipins 12-oxophytodienoic acid jasmonic acid Abscisic Acid
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Dave Anuja
Centre for Novel Agricultural Products, Department of Biology, University of York, Heslington, York YO105DD, United Kingdom.
Hernández M Luisa
He Zhesi
Andriotis Vasilios M E
Vaistij Fabián E
Larson Tony R
Graham Ian A
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.110.081489
Published
2011-02-00
Epub
2011-00-18
Pages
583-99
Language
English
Region
England
NLM ID
9208688
PMCID
PMC3077774
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BB/D006856/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · BB/E022081/1 · United Kingdom
External Links
PubMed source DOI: 10.1105/tpc.110.081489 PMC Full Text View journal details
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