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PMID: 21343414 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Delivery of prolamins to the protein storage vacuole in maize aleurone cells.

The Plant cell ·Vol. 23 ·No. 2 ·2011-02-00 ·Pages 769-84

Reyes FC, Chung T, Holding D, Jung R, Vierstra R, Otegui MS

Abstract

Zeins, the prolamin storage proteins found in maize (Zea mays), accumulate in accretions called protein bodies inside the endoplasmic reticulum (ER) of starchy endosperm cells. We found that genes encoding zeins, α-globulin, and legumin-1 are transcribed not only in the starchy endosperm but also in aleurone cells. Unlike the starchy endosperm, aleurone cells accumulate these storage proteins inside protein storage vacuoles (PSVs) instead of the ER. Aleurone PSVs contain zein-rich protein inclusions, a matrix, and a large system of intravacuolar membranes. After being assembled in the ER, zeins are delivered to the aleurone PSVs in atypical prevacuolar compartments that seem to arise at least partially by autophagy and consist of multilayered membranes and engulfed cytoplasmic material. The zein-containing prevacuolar compartments are neither surrounded by a double membrane nor decorated by AUTOPHAGY RELATED8 protein, suggesting that they are not typical autophagosomes. The PSV matrix contains glycoproteins that are trafficked through a Golgi-multivesicular body (MVB) pathway. MVBs likely fuse with the multilayered, autophagic compartments before merging with the PSV. The presence of similar PSVs also containing prolamins and large systems of intravacuolar membranes in wheat (Triticum aestivum) and barley (Hordeum vulgare) starchy endosperm suggests that this trafficking mechanism may be common among cereals.

MeSH Terms
Electron Microscope Tomography Endosperm/metabolism Gene Expression Profiling Gene Expression Regulation, Plant Golgi Apparatus/metabolism Microscopy, Confocal Prolamins/genetics,metabolism Protein Transport Seeds/metabolism Vacuoles/metabolism Zea mays/genetics,metabolism Zein/genetics,metabolism
Chemicals
Prolamins Zein
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Reyes Francisca C
Department of Botany, University of Wisconsin, Madison, Wisconsin 53706, USA.
Chung Taijoon
Holding David
Jung Rudolf
Vierstra Richard
Otegui Marisa S
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.110.082156
Published
2011-02-00
Epub
2011-00-22
Pages
769-84
Language
English
Region
England
NLM ID
9208688
PMCID
PMC3077793
Subset
IM
Corrections
CommentIn
PMID: 22180629
External Links
PubMed source DOI: 10.1105/tpc.110.082156 PMC Full Text View journal details
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