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PMID: 21357674 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Autoregulation of convergent RNAi genes in fission yeast.

Genes & development ·Vol. 25 ·No. 6 ·2011-03-15 ·Pages 556-68

Gullerova M, Moazed D, Proudfoot NJ

Abstract

RNAi plays a central role in the regulation of eukaryotic genes. In Schizosaccharomyces pombe fission yeast, RNAi involves the formation of siRNA from dsRNA that acts to establish and maintain heterochromatin over centromeres, telomeres, and mating loci. We showed previously that transient heterochromatin also forms over S. pombe convergent genes (CGs). Remarkably, most RNAi genes are themselves convergent. We demonstrate here that transient heterochromatin formed by the RNAi pathway over RNAi CGs leads to their autoregulation in G1-S. Furthermore, the switching of RNAi gene orientation from convergent to tandem causes loss of their G1-S down-regulation. Surprisingly, yeast mutants with tandemized dcr1, ago1, or clr4 genes display aberrant centromeric heterochromatin, which results in abnormal cell morphology. Our results emphasize the significance of gene orientation for correct RNAi gene expression, and suggest a role for cell cycle-dependent formation of RNAi CG heterochromatin in cellular integrity.

MeSH Terms
Cell Cycle Centromere/metabolism Gene Expression Regulation, Fungal Gene Order/genetics Gene Silencing Heterochromatin Mutation Phenotype RNA Interference Schizosaccharomyces/genetics,metabolism Schizosaccharomyces pombe Proteins/metabolism
Chemicals
Heterochromatin Schizosaccharomyces pombe Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gullerova Monika
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.
Moazed Danesh
Proudfoot Nick J
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
1549-5477
DOI
10.1101/gad.618611
Published
2011-03-15
Epub
2011-00-28
Pages
556-68
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC3059830
Subset
IM
Grants
Cancer Research UK · United Kingdom
Howard Hughes Medical Institute · United States
Wellcome Trust · United Kingdom
Corrections
CommentIn
PMID: 21406552
External Links
PubMed source DOI: 10.1101/gad.618611 PMC Full Text View journal details
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