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PMID: 2136767 Published · ppublish English Journal Article

Molecular cloning of Xenopus fibrillarin, a conserved U3 small nuclear ribonucleoprotein recognized by antisera from humans with autoimmune disease.

Molecular and cellular biology ·Vol. 10 ·No. 1 ·1990-01-00 ·Pages 430-4

Lapeyre B, Mariottini P, Mathieu C, Ferrer P, Amaldi F, Amalric F, Caizergues-Ferrer M

Abstract

Autoantibodies against U3 small nuclear ribonucleoprotein are associated with scleroderma autoimmune disease. They were shown to react with fibrillarin, a 34- to 36-kilodalton protein that has been detected in all eukaryotes tested from humans to yeasts. We isolated a 1.6-kilobase cDNA encoding fibrillarin from a Xenopus laevis cDNA library. The protein contains a 79-residue-long Gly-Arg-rich domain in its N-terminal region and a putative RNA-binding domain with ribonucleoprotein consensus sequence in its central portion. This is the first report of cloning of fibrillarin, and the deduced protein sequence is in agreement with the involvement of the protein in a ribonucleoprotein particle.

MeSH Terms
Amino Acid Sequence Animals Autoantigens/immunology Autoimmune Diseases/immunology Base Sequence Binding Sites Blotting, Northern Chromosomal Proteins, Non-Histone/genetics Cloning, Molecular Molecular Sequence Data RNA, Small Nuclear/metabolism Recombinant Proteins Ribonucleoproteins/genetics Ribonucleoproteins, Small Nuclear Xenopus laevis/genetics
Chemicals
Autoantigens Chromosomal Proteins, Non-Histone RNA, Small Nuclear Recombinant Proteins Ribonucleoproteins Ribonucleoproteins, Small Nuclear fibrillarin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lapeyre B
Centre de Recherche de Biochimie, Centre National de la Recherche Scientifique, Toulouse, France.
Mariottini P
Mathieu C
Ferrer P
Amaldi F
Amalric F
Caizergues-Ferrer M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.1.430-434.1990
Published
1990-01-00
Pages
430-4
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360777
Subset
IM
Databases
GENBANK
M28874
External Links
PubMed source DOI: 10.1128/mcb.10.1.430-434.1990 PMC Full Text View journal details
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