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PMID: 21368773 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Peripheral blood gene expression profiles in metabolic syndrome, coronary artery disease and type 2 diabetes.

Genes and immunity ·Vol. 12 ·No. 5 ·2011-07-00 ·Pages 341-51

Grayson BL, Wang L, Aune TM

Abstract

To determine if individuals with metabolic disorders possess unique gene expression profiles, we compared transcript levels in peripheral blood from patients with coronary artery disease (CAD), type 2 diabetes (T2D) and their precursor state, metabolic syndrome to those of control (CTRL) subjects and subjects with rheumatoid arthritis (RA). The gene expression profile of each metabolic state was distinguishable from CTRLs and correlated with other metabolic states more than with RA. Of note, subjects in the metabolic cohorts overexpressed gene sets that participate in the innate immune response. Genes involved in activation of the pro-inflammatory transcription factor, NF-κB, were overexpressed in CAD whereas genes differentially expressed in T2D have key roles in T-cell activation and signaling. Reverse transcriptase PCR validation confirmed microarray results. Furthermore, several genes differentially expressed in human metabolic disorders have been previously shown to participate in inflammatory responses in murine models of obesity and T2D. Taken together, these data demonstrate that peripheral blood from individuals with metabolic disorders display overlapping and non-overlapping patterns of gene expression indicative of unique, underlying immune processes.

MeSH Terms
Arthritis, Rheumatoid/genetics Cluster Analysis Coronary Artery Disease/genetics Diabetes Mellitus, Type 2/genetics Gene Expression Profiling Gene Expression Regulation Genetic Predisposition to Disease/genetics Humans Metabolic Syndrome/genetics Polymorphism, Single Nucleotide/genetics Reproducibility of Results
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Grayson B L
Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
Wang L
Aune T M
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Article Info
Journal
Genes and immunity
Abbr.
Genes Immun
ISSN
1476-5470
DOI
10.1038/gene.2011.13
Published
2011-07-00
Epub
2011-00-03
Pages
341-51
Language
English
Region
England
NLM ID
100953417
PMCID
PMC3137736
Subset
IM
Grants
NIDDK NIH HHS · R42 DK065388 · United States
NIDDK NIH HHS · T32 DK07563 · United States
NIGMS NIH HHS · T32 GM07347 · United States
NCRR NIH HHS · TL1 RR024978 · United States
NIAID NIH HHS · R42 AI053984 · United States
NIAID NIH HHS · R42 AI053984-06 · United States
NIGMS NIH HHS · T32 GM007347 · United States
NIDDK NIH HHS · R42 DK065388-03 · United States
NIDDK NIH HHS · T32 DK007563 · United States
External Links
PubMed source DOI: 10.1038/gene.2011.13 PMC Full Text View journal details
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