Purification, cloning, expression and biological characterization of an interleukin-1 receptor antagonist protein.

Nature ·Vol. 344 ·No. 6267 ·1990-04-12 ·Pages 633-8

Carter DB, Deibel MR, Dunn CJ, Tomich CS, Laborde AL, Slightom JL, Berger AE, Bienkowski MJ, Sun FF, McEwan RN

Abstract

A human myelomonocytic cell line, U937, produced an interleukin-1 (IL-1) receptor antagonist protein (IRAP) which was purified and partially sequenced. A complementary DNA coding for IRAP was cloned and sequenced. The mature translation product of the cDNA has been expressed in Escherichia coli and was an active competitive inhibitor of the binding of IL-1 to the T-cell/fibroblast form of the IL-1 receptor. Recombinant IRAP specifically inhibited IL-1 bioactivity on T cells and endothelial cells in vitro and was a potent inhibitor of IL-1 induced corticosterone production in vivo.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Binding, Competitive Cell Adhesion Cell Line Cloning, Molecular Colony-Stimulating Factors/pharmacology Corticosterone/blood DNA/genetics Gene Expression Granulocyte-Macrophage Colony-Stimulating Factor Granulocytes/metabolism Growth Substances/pharmacology Humans Interleukin 1 Receptor Antagonist Protein Interleukin-1/metabolism,pharmacology Mice Molecular Sequence Data Monocytes/metabolism Neutrophils/cytology,physiology Proteins/genetics,isolation & purification,pharmacology Receptors, Immunologic/antagonists & inhibitors,metabolism Receptors, Interleukin-1 Recombinant Proteins Sialoglycoproteins Tetradecanoylphorbol Acetate/pharmacology

Chemicals

Colony-Stimulating Factors Growth Substances IL1RN protein, human Il1rn protein, mouse Interleukin 1 Receptor Antagonist Protein Interleukin-1 Proteins Receptors, Immunologic Receptors, Interleukin-1 Recombinant Proteins Sialoglycoproteins Granulocyte-Macrophage Colony-Stimulating Factor DNA Tetradecanoylphorbol Acetate Corticosterone

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Carter D B

Department of Molecular Biology Research, Upjohn Company, Kalamazoo, Michigan 49007.

Deibel M R

Dunn C J

Tomich C S

Laborde A L

Slightom J L

Berger A E

Bienkowski M J

Sun F F

McEwan R N

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/344633a0

Published

1990-04-12

Pages

633-8

Language

English

Region

England

NLM ID

0410462

Subset

Corrections

CommentIn

PMID: 2139179

External Links

PubMed source DOI: 10.1038/344633a0 View journal details

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Purification, cloning, expression and biological characterization of an interleukin-1 receptor antagonist protein.

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