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PMID: 2140357 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Regulatory proteins of F1F0-ATPase: role of ATPase inhibitor.

Journal of bioenergetics and biomembranes ·Vol. 22 ·No. 1 ·1990-02-00 ·Pages 27-38

Hashimoto T, Yoshida Y, Tagawa K

Abstract

An intrinsic ATPase inhibitor inhibits the ATP-hydrolyzing activity of mitochondrial F1F0-ATPase and is released from its binding site on the enzyme upon energization of mitochondrial membranes to allow phosphorylation of ADP. The mitochondrial activity to synthesize ATP is not influenced by the absence of the inhibitor protein. The enzyme activity to hydrolyze ATP is induced by dissipation of the membrane potential in the absence of the inhibitor. Thus, the inhibitor is not responsible for oxidative phosphorylation, but acts only to inhibit ATP hydrolysis by F1F0-ATPase upon deenergization of mitochondrial membranes. The inhibitor protein forms a regulatory complex with two stabilizing factors, 9K and 15K proteins, which facilitate the binding of the inhibitor to F1F0-ATPase and stabilize the resultant inactivated enzyme. The 9K protein, having a sequence very similar to the inhibitor, binds directly to F1 in a manner similar to the inhibitor. The 15K protein binds to the F0 part and holds the inhibitor and the 9K protein on F1F0-ATPase even when one of them is detached from the F1 part.

MeSH Terms
Adenosine Diphosphate/metabolism Adenosine Triphosphate/metabolism Amino Acid Sequence Escherichia coli/enzymology Mitochondria/enzymology Molecular Sequence Data Phosphorylation Proteins/metabolism Proton-Translocating ATPases/antagonists & inhibitors,metabolism Sequence Homology, Nucleic Acid Yeasts/enzymology
Chemicals
ATPase inhibitory protein Proteins Adenosine Diphosphate Adenosine Triphosphate Proton-Translocating ATPases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hashimoto T
Department of Physiological Chemistry, Medical School, Osaka University, Japan.
Yoshida Y
Tagawa K
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Article Info
Journal
Journal of bioenergetics and biomembranes
Abbr.
J Bioenerg Biomembr
ISSN
0145-479X
DOI
10.1007/BF00762843
Published
1990-02-00
Pages
27-38
Language
English
Region
United States
NLM ID
7701859
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00762843 View journal details
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