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PMID: 21439473 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Structural basis of cooperative ligand binding by the glycine riboswitch.

Chemistry & biology ·Vol. 18 ·No. 3 ·2011-03-25 ·Pages 293-8

Butler EB, Xiong Y, Wang J, Strobel SA

Abstract

The glycine riboswitch regulates gene expression through the cooperative recognition of its amino acid ligand by a tandem pair of aptamers. A 3.6 Å crystal structure of the tandem riboswitch from the glycine permease operon of Fusobacterium nucleatum reveals the glycine binding sites and an extensive network of interactions, largely mediated by asymmetric A-minor contacts, that serve to communicate ligand binding status between the aptamers. These interactions provide a structural basis for how the glycine riboswitch cooperatively regulates gene expression.

MeSH Terms
Aptamers, Nucleotide/chemistry,metabolism Binding Sites Gene Expression Regulation Glycine/chemistry Ligands Nucleic Acid Conformation RNA/chemistry Riboswitch
Chemicals
Aptamers, Nucleotide Ligands Riboswitch RNA Glycine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Butler Ethan B
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA.
Xiong Yong
Wang Jimin
Strobel Scott A
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Article Info
Journal
Chemistry & biology
Abbr.
Chem Biol
ISSN
1879-1301
DOI
10.1016/j.chembiol.2011.01.013
Published
2011-03-25
Pages
293-8
Language
English
Region
United States
NLM ID
9500160
PMCID
PMC3076126
Subset
IM
Grants
NIGMS NIH HHS · GM02278 · United States
NIGMS NIH HHS · P01 GM022778 · United States
NIGMS NIH HHS · P01 GM022778-34 · United States
NIGMS NIH HHS · P01 GM022778-35 · United States
NIGMS NIH HHS · P01 GM022778-36 · United States
NIBIB NIH HHS · P30 EB009998 · United States
Databases
PDB
3P49
External Links
PubMed source DOI: 10.1016/j.chembiol.2011.01.013 PMC Full Text
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