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PMID: 2144903 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A cloned DNA segment from the telomeric region of human chromosome 4p is not detectably rearranged in Huntington disease patients.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 18 ·1990-09-00 ·Pages 7309-13

Pritchard C, Casher D, Bull L, Cox DR, Myers RM

Abstract

Genetic linkage studies have mapped the Huntington disease (HD) mutation to the distal region of the short arm of human chromosome 4. Analysis of recombination events in this region has produced contradictory locations for HD. One possible location is in the region distal to the D4S90 marker, which is located within 300 kilobases of the telomere. Other crossover events predict a more centromeric position for HD. Here we analyze the telomeric region of 4p in detail. Cloned DNA segments were derived from this region by utilizing a radiation-induced somatic cell hybrid as a source of DNA combined with preparative pulsed-field gel electrophoresis to enrich for the telomeric fraction. Additional DNA was obtained by using the cloned segments as multiple start points for cosmid walks. This strategy proved to be an effective method for cloning 250 kilobases of DNA in the region telomeric to D4S90. Hybridization analysis with the cloned DNA did not provide any evidence for the presence of rearrangements of 100 base pairs or greater in the DNA of individuals affected with HD. We also found no change in the size or structure of the 4p telomere in these samples.

MeSH Terms
Animals Cell Line Chromosome Mapping Chromosomes, Human, Pair 4 Cloning, Molecular DNA/genetics,isolation & purification Gene Rearrangement Genetic Linkage Humans Huntington Disease/genetics Hybrid Cells/cytology Mice Nucleic Acid Hybridization Restriction Mapping
Chemicals
DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Pritchard C
Department of Physiology, University of California, San Francisco 94143.
Casher D
Bull L
Cox D R
Myers R M
References (23)
23 references, click to expand
  1. Recombination events suggest potential sites for the Huntington's disease gene.
    Neuron. 1989 Aug;3(2):183-90 PMID: 2576211
  2. Hypervariable telomeric sequences from the human sex chromosomes are pseudoautosomal.
    Nature. 1985 Oct 24-30;317(6039):687-92 PMID: 2997619
  3. Cloning of human telomeres by complementation in yeast.
    Nature. 1989 Apr 27;338(6218):771-4 PMID: 2541341
  4. Mapping of D4S98/S114/S113 confines the Huntington's defect to a reduced physical region at the telomere of chromosome 4.
    Nucleic Acids Res. 1988 Dec 23;16(24):11769-80 PMID: 2905444
  5. Derivation of clones close to met by preparative field inversion gel electrophoresis.
    Science. 1987 Jun 5;236(4806):1305-8 PMID: 3035716
  6. Physical maps of 4p16.3, the area expected to contain the Huntington disease mutation.
    Genomics. 1990 Jan;6(1):1-15 PMID: 2137426
  7. A polymorphic DNA marker genetically linked to Huntington's disease.
    Nature. 1983 Nov 17-23;306(5940):234-8 PMID: 6316146
  8. Isolation of DNA markers in the direction of the Huntington disease gene from the G8 locus.
    Am J Hum Genet. 1988 Feb;42(2):335-44 PMID: 2963539
  9. Localization of the Huntington's disease gene to a small segment of chromosome 4 flanked by D4S10 and the telomere.
    Cell. 1987 Aug 14;50(4):565-71 PMID: 2886227
  10. A yeast artificial chromosome telomere clone spanning a possible location of the Huntington disease gene.
    Am J Hum Genet. 1990 Apr;46(4):762-75 PMID: 2138410
  11. The Huntington disease locus is most likely within 325 kilobases of the chromosome 4p telomere.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):10011-4 PMID: 2557612
  12. Molecular Genetics of the Bithorax Complex in Drosophila melanogaster.
    Science. 1983 Jul 1;221(4605):23-9 PMID: 17737996
  13. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.
    Science. 1987 May 15;236(4803):806-12 PMID: 3033825
  14. High efficiency vectors for cosmid microcloning and genomic analysis.
    Gene. 1989 Jun 30;79(1):9-20 PMID: 2777090
  15. Separation of large DNA molecules by contour-clamped homogeneous electric fields.
    Science. 1986 Dec 19;234(4783):1582-5 PMID: 3538420
  16. Segregation of the Huntington disease region of human chromosome 4 in a somatic cell hybrid.
    Genomics. 1989 Apr;4(3):397-407 PMID: 2523853
  17. Huntington disease: no evidence for locus heterogeneity.
    Genomics. 1989 Aug;5(2):304-8 PMID: 2571579
  18. Evidence from family studies that the gene causing Huntington disease is telomeric to D4S95 and D4S90.
    Am J Hum Genet. 1989 Mar;44(3):422-5 PMID: 2521771
  19. Non-random association between alleles detected at D4S95 and D4S98 and the Huntington's disease gene.
    J Med Genet. 1989 Nov;26(11):676-81 PMID: 2531224
  20. A somatic cell hybrid panel for localizing DNA segments near the Huntington's disease gene.
    Genomics. 1987 Sep;1(1):29-34 PMID: 2889660
  21. Isolation and field-inversion gel electrophoresis analysis of DNA markers located close to the Huntington disease gene.
    Genomics. 1989 Apr;4(3):408-18 PMID: 2523854
  22. Linkage disequilibrium in Huntington's disease: an improved localisation for the gene.
    J Med Genet. 1989 Nov;26(11):673-5 PMID: 2531223
  23. Molecular cloning of human telomeres in yeast.
    Nature. 1989 Apr 27;338(6218):774-6 PMID: 2541342
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.18.7309
Published
1990-09-00
Pages
7309-13
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54733
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.18.7309 PMC Full Text View journal details
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