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PMID: 2145150 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A feedback control element near the transcription start site of the maize Shrunken gene determines promoter activity.

The EMBO journal ·Vol. 9 ·No. 11 ·1990-11-00 ·Pages 3447-52

Maas C, Schaal S, Werr W

Abstract

The transcriptional activity of the Shrunken (Sh) promoter of Zea mays was monitored in transient expression assays using the neomycin phosphotransferase (NPT) II gene as a reporter in maize suspension protoplasts. Shortly after transfection, expression of this chimeric NPTII gene was negatively affected by high extracellular sucrose concentrations in the protoplast cultivation medium. However, 3-5 days after transfection an up to 405-fold increase in NPTII activity was observed. This could be blocked by dichlorobenzonitril (DCB) an inhibitor of cellulose biosynthesis. In the analysis of promoter deletions 20 bp upstream of the Sh transcription start site were sufficient to reproduce the expression profile and the activity of the full promoter. Surprisingly this start sequence does not include the natural TATA-box.

Related Genes
Sh
MeSH Terms
Base Sequence Cell Wall/metabolism Cells, Cultured Cloning, Molecular Feedback Gene Expression Regulation/drug effects Glucosyltransferases/genetics Molecular Sequence Data Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Sucrose/pharmacology Transcription, Genetic/drug effects Zea mays/genetics
Chemicals
Sucrose Glucosyltransferases sucrose synthase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Maas C
Institut für Genetik, Universität zu Köln Weyertal, FRG.
Schaal S
Werr W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-11-00
Pages
3447-52
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552092
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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