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PMID: 2145151 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The nematode spliced leader RNA participates in trans-splicing as an Sm snRNP.

The EMBO journal ·Vol. 9 ·No. 11 ·1990-11-00 ·Pages 3667-73

Maroney PA, Hannon GJ, Denker JA, Nilsen TW

Abstract

The trans-spliced leader RNA (SL RNA) of nematodes resembles U snRNAs both in cap structure and in the presence of a consensus Sm binding site. We show here that synthetic SL RNA, synthesized by in vitro transcription, is efficiently used as a spliced leader donor in trans-splicing reactions catalyzed by a cell free extract prepared from developing embryos of the parasitic nematode, Ascaris lumbricoides. Efficient utilization of synthetic SL RNA requires a functional Sm binding site. Mutations within the Sm binding sequence that prevent immunoprecipitation by Sm antisera and prevent cap trimethylation abolish trans-splicing. The effect on trans-splicing is not due to undermethylation of the cap structure.

MeSH Terms
Animals Ascaris/genetics Autoantigens/physiology Base Sequence Binding Sites DNA Mutational Analysis In Vitro Techniques Molecular Sequence Data Nucleic Acid Precursors/metabolism Oligonucleotides RNA Caps RNA Splicing RNA, Messenger/metabolism Ribonucleoproteins/physiology Ribonucleoproteins, Small Nuclear snRNP Core Proteins
Chemicals
Autoantigens Nucleic Acid Precursors Oligonucleotides RNA Caps RNA, Messenger Ribonucleoproteins Ribonucleoproteins, Small Nuclear snRNP Core Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Maroney P A
Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH 44106.
Hannon G J
Denker J A
Nilsen T W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-11-00
Pages
3667-73
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552121
Subset
IM
Grants
NIGMS NIH HHS · GM31528 · United States
External Links
PubMed source PMC Full Text View journal details
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