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PMID: 21454474 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Vital roles of the second DNA-binding site of Rad52 protein in yeast homologous recombination.

The Journal of biological chemistry ·Vol. 286 ·No. 20 ·2011-05-20 ·Pages 17607-17

Arai N, Kagawa W, Saito K, Shingu Y, Mikawa T, Kurumizaka H, Shibata T

Abstract

RecA/Rad51 proteins are essential in homologous DNA recombination and catalyze the ATP-dependent formation of D-loops from a single-stranded DNA and an internal homologous sequence in a double-stranded DNA. RecA and Rad51 require a "recombination mediator" to overcome the interference imposed by the prior binding of single-stranded binding protein/replication protein A to the single-stranded DNA. Rad52 is the prototype of recombination mediators, and the human Rad52 protein has two distinct DNA-binding sites: the first site binds to single-stranded DNA, and the second site binds to either double- or single-stranded DNA. We previously showed that yeast Rad52 extensively stimulates Rad51-catalyzed D-loop formation even in the absence of replication protein A, by forming a 2:1 stoichiometric complex with Rad51. However, the precise roles of Rad52 and Rad51 within the complex are unknown. In the present study, we constructed yeast Rad52 mutants in which the amino acid residues corresponding to the second DNA-binding site of the human Rad52 protein were replaced with either alanine or aspartic acid. We found that the second DNA-binding site is important for the yeast Rad52 function in vivo. Rad51-Rad52 complexes consisting of these Rad52 mutants were defective in promoting the formation of D-loops, and the ability of the complex to associate with double-stranded DNA was specifically impaired. Our studies suggest that Rad52 within the complex associates with double-stranded DNA to assist Rad51-mediated homologous pairing.

MeSH Terms
Binding Sites DNA, Fungal/genetics,metabolism Humans Multiprotein Complexes/genetics,metabolism Mutation Rad51 Recombinase/genetics,metabolism Rad52 DNA Repair and Recombination Protein/genetics,metabolism Recombination, Genetic/physiology Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Sequence Homology, Amino Acid
Chemicals
DNA, Fungal Multiprotein Complexes RAD52 protein, S cerevisiae Rad52 DNA Repair and Recombination Protein Saccharomyces cerevisiae Proteins RAD51 protein, S cerevisiae Rad51 Recombinase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Arai Naoto
Department of Applied Biological Science, Nihon University College of Bioresource Sciences, Fujisawa-shi, Kanagawa 252-0880, Japan. [email protected]
Kagawa Wataru
Saito Kengo
Shingu Yoshinori
Mikawa Tsutomu
Kurumizaka Hitoshi
Shibata Takehiko
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
1083-351X
DOI
10.1074/jbc.M110.216739
Email
[email protected]
Published
2011-05-20
Epub
2011-00-28
Pages
17607-17
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC3093836
Subset
IM
External Links
PubMed source DOI: 10.1074/jbc.M110.216739 PMC Full Text View journal details
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