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PMID: 214758 Published · ppublish English Journal Article

SV40 viral minichromosome: preferential exposure of the origin of replication as probed by restriction endonucleases.

Nucleic acids research ·Vol. 5 ·No. 10 ·1978-10-00 ·Pages 3469-77

Varshavsky AJ, Sundin OH, Bohn MJ

Abstract

Isolated SV40 minichromosomes [1-3] were treated with different single-cut restriction endonucleases to probe the arrangement of nucleosomes in relation to the SV70 DNA sequence. While Eco RI and Bam HI each cut 22-27% of the SV40 minichromosomes under limit-digest conditions, Bgl I, which cuts SV40 DNA at or very near the origin of replication [4,5], cleaves 90-95% of the minichromosomes in a preparation. Similar results were obtained with minichromosomes which had been fixed with formaldehyde before endonuclease treatment. One possible interpretation of these findings is that the arrangement of nucleosomes in the compact SV40 minichromosomes is nonrandom at least with regard to sequences near the origin of DNA replication.

MeSH Terms
Binding Sites Chemical Phenomena Chemistry Chromatin/ultrastructure DNA Replication DNA Restriction Enzymes/metabolism Formaldehyde Histones/metabolism Protein Binding Simian virus 40/genetics,ultrastructure Virus Replication
Chemicals
Chromatin Histones Formaldehyde DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Varshavsky A J
Sundin O H
Bohn M J
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25 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.10.3469
Published
1978-10-00
Pages
3469-77
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342688
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.10.3469 PMC Full Text View journal details
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