Quantitative determination of the lateral diffusion coefficients of the hormone-receptor complexes of insulin and epidermal growth factor on the plasma membrane of cultured fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 11 ·1978-11-00 ·Pages 5353-7

Schlessinger J, Shechter Y, Cuatrecasas P, Willingham MC, Pastan I

Abstract

Fluorescent derivatives of insulin and epidermal growth factor bound to 3T3 mouse fibroblasts are mobile on the cell surface, with similar diffusion coefficients, D approximately (3--5) x 10(-10) cm2/sec at 23 degrees C. Increasing the temperature to 37 degrees C results in rapid receptor immobilization. The immobilization is attributed to aggregation of hormone-receptor complexes, their internalization, or a combination of both processes.

MeSH Terms

Azides/pharmacology Binding, Competitive Cell Line Cell Membrane/metabolism Epidermal Growth Factor/metabolism Fibroblasts/metabolism Fluorescent Dyes Insulin/metabolism Kinetics Peptides/metabolism Receptor, Insulin/metabolism Receptors, Cell Surface/metabolism

Chemicals

Azides Fluorescent Dyes Insulin Peptides Receptors, Cell Surface Epidermal Growth Factor Receptor, Insulin

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Schlessinger J

Shechter Y

Cuatrecasas P

Willingham M C

Pastan I

References (27)

27 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.11.5353

Published

1978-11-00

Pages

5353-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC392961

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.11.5353 PMC Full Text View journal details

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Quantitative determination of the lateral diffusion coefficients of the hormone-receptor complexes of insulin and epidermal growth factor on the plasma membrane of cultured fibroblasts.

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