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PMID: 21483813 Published · epublish English Journal Article

The accumulation and prognosis value of tumor infiltrating IL-17 producing cells in esophageal squamous cell carcinoma.

PloS one ·Vol. 6 ·No. 3 ·2011-03-31 ·Pages e18219

Lv L, Pan K, Li XD, She KL, Zhao JJ, Wang W, Chen JG, Chen YB, Yun JP, Xia JC

Abstract

The role of IL-17 producing cells in tumors is controversial. In the present study, we investigated the prognostic value of measuring tumor-infiltrating IL-17 producing cell levels in human esophageal squamous cell carcinoma (ESCC). Immunohistochemical staining was performed to investigate the levels of IL-17+ tumor infiltrating lymphocytes (TILs), as well as CD8+ cytotoxic T lymphocytes (CTLs) and CD57+ natural killer (NK) cells from 181 ESCC patients. The prognostic value of measuring the densities of IL-17+TILs and the correlation with CTLs and NK was evaluated. IL-17 producing cells were detected in esophageal squamous cell carcinoma tissues. The IL-17 producing cells were major CD4 positive, but Foxp3 negative. The median level of IL-17+TILs was 3.90 cells/high power microscopic field (HPF). The density of IL-17 producing cells correlated negatively with T stage (P=0.042). The higher densities of tumor infiltrating IL-17+ lymphocytes were associated with better overall survival (P=0.031). Furthermore, we found that there were positive correlations between levels of IL-17 producing cells and the densities of CD8+cells, as well as CD57+cells (r=0.198, P=0.008 for CD8+ cells and r=0.261, P<0.001 for CD57+ cells, respectively). The prognosis analysis also showed that the higher levels of CD8+ CTLs and CD57+ NK cells correlated with better overall survival of ESCC patients. Our study suggests that tumor infiltrating IL-17 producing cells in ESCC patients may have protective roles in the tumor microenvironment and may be treated as a prognostic marker for ESCC patients.

MeSH Terms
Adult Aged CD57 Antigens/metabolism CD8-Positive T-Lymphocytes/immunology,metabolism Carcinoma, Squamous Cell/immunology,pathology Esophageal Neoplasms/immunology,pathology Female Humans Immunohistochemistry In Vitro Techniques Interleukin-17/metabolism Killer Cells, Natural/immunology,metabolism Lymphocytes, Tumor-Infiltrating/immunology,metabolism Male Middle Aged
Chemicals
CD57 Antigens Interleukin-17
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Lv Lin
State Key Laboratory of Oncology in Southern China and Department of Experimental Research, Cancer Center, Sun Yat-sen University, Guangzhou, People's Republic of China.
Pan Ke
Li Xiao-dong
She Ke-lin
Zhao Jing-jing
Wang Wei
Chen Ju-gao
Chen Yi-bin
Yun Jing-ping
Xia Jian-chuan
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0018219
Published
2011-03-31
Epub
2011-00-31
Pages
e18219
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3069054
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0018219 PMC Full Text View journal details
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