Acid elution of blood group antibodies from intact erythrocytes.

Vox sanguinis ·Vol. 33 ·No. 5 ·1977-00-00 ·Pages 280-5

Rekvig OP, Hannestad K

Abstract

Human IgM and IgG antibodies against blood group antigens (A, B, D, C, c, E, e, Fya, K), autoantibodies and mouse IgM and IgG antibodies against sheep erythrocytes have been eluted from intact human and sheep red cells by glycine-HCl buffer, pH 3.0. The yield of human antibodies was higher with acid than with heat and ether elution, and the contamination of hemoglobin in the eluate was negligible. The acid elution method is very simple and rapid and, therefore, highly suitable for experimental as well as routine immunohematological work.

MeSH Terms

ABO Blood-Group System Animals Blood Group Antigens Chromatography, Gel Erythrocytes/immunology Glycine/metabolism Goats Humans Hydrochloric Acid/metabolism Hydrogen-Ion Concentration Immunoglobulin G/analysis Immunoglobulin M/analysis Immunologic Techniques Isoantibodies Mice Rh-Hr Blood-Group System Sheep Temperature Time Factors

Chemicals

ABO Blood-Group System Blood Group Antigens Immunoglobulin G Immunoglobulin M Isoantibodies Rh-Hr Blood-Group System Hydrochloric Acid Glycine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Rekvig O P

Hannestad K

Article Info

Journal

Vox sanguinis

Abbr.

Vox Sang

ISSN

0042-9007

DOI

10.1111/j.1423-0410.1977.tb04476.x

Published

1977-00-00

Pages

280-5

Language

English

Region

England

NLM ID

0413606

Subset

External Links

PubMed source DOI: 10.1111/j.1423-0410.1977.tb04476.x View journal details

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Acid elution of blood group antibodies from intact erythrocytes.

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