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PMID: 21491967 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Review

The multiparametric effects of hydrodynamic environments on stem cell culture.

Tissue engineering. Part B, Reviews ·Vol. 17 ·No. 4 ·2011-08-00 ·Pages 249-62

Kinney MA, Sargent CY, McDevitt TC

Abstract

Stem cells possess the unique capacity to differentiate into many clinically relevant somatic cell types, making them a promising cell source for tissue engineering applications and regenerative medicine therapies. However, in order for the therapeutic promise of stem cells to be fully realized, scalable approaches to efficiently direct differentiation must be developed. Traditionally, suspension culture systems are employed for the scale-up manufacturing of biologics via bioprocessing systems that heavily rely upon various types of bioreactors. However, in contrast to conventional bench-scale static cultures, large-scale suspension cultures impart complex hydrodynamic forces on cells and aggregates due to fluid mixing conditions. Stem cells are exquisitely sensitive to environmental perturbations, thus motivating the need for a more systematic understanding of the effects of hydrodynamic environments on stem cell expansion and differentiation. This article discusses the interdependent relationships between stem cell aggregation, metabolism, and phenotype in the context of hydrodynamic culture environments. Ultimately, an improved understanding of the multifactorial response of stem cells to mixed culture conditions will enable the design of bioreactors and bioprocessing systems for scalable directed differentiation approaches.

MeSH Terms
Cell Culture Techniques/methods Cells, Cultured Humans Hydrodynamics Stem Cells/cytology,metabolism
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kinney Melissa A
The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332-0532, USA.
Sargent Carolyn Y
McDevitt Todd C
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Article Info
Journal
Tissue engineering. Part B, Reviews
Abbr.
Tissue Eng Part B Rev
ISSN
1937-3376
DOI
10.1089/ten.TEB.2011.0040
Published
2011-08-00
Epub
2011-00-25
Pages
249-62
Language
English
Region
United States
NLM ID
101466660
PMCID
PMC3142632
Subset
IM
Grants
NIBIB NIH HHS · EB010061 · United States
External Links
PubMed source DOI: 10.1089/ten.TEB.2011.0040 PMC Full Text View journal details
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