Home LiteratureArticle Details
PMID: 2154681 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The PRP4 (RNA4) protein of Saccharomyces cerevisiae is associated with the 5' portion of the U4 small nuclear RNA.

Molecular and cellular biology ·Vol. 10 ·No. 3 ·1990-03-00 ·Pages 1217-25

Xu Y, Petersen-Bjørn S, Friesen JD

Abstract

We have combined oligonucleotide-directed RNase H degradation and immunoprecipitation in a study of the association of the Saccharomyces cerevisiae PRP4 protein with the U4-U6 complex. We have found that three oligonucleotides were able to direct nearly to completion the RNase H-specific cleavage of the target RNA molecules as they exist in splicing extracts. Immunoprecipitation of the degradation products with PRP4 antibody showed that the 5' portion of U4 small nuclear RNA (snRNA) and the 3' portion of U6 snRNA coimmunoprecipitated with the PRP4 protein. Micrococcal nuclease protection experiments confirmed further that the 5' portion and 3' end of U4 snRNA were very resistant to nuclease digestion, whereas the 3' portion of U6 snRNA was protected to only a very small extent. We conclude that the PRP4 protein of S. cerevisiae is associated primarily with the 5' portion of U4 snRNA in the U4-U6 small nuclear ribonucleoprotein (snRNP).

MeSH Terms
Base Sequence Endoribonucleases/pharmacology Fungal Proteins/metabolism Molecular Sequence Data Oligonucleotide Probes Oligoribonucleotides Precipitin Tests RNA Splicing RNA, Fungal/metabolism RNA, Small Nuclear/metabolism,ultrastructure Ribonuclease H Ribonucleoproteins/metabolism Saccharomyces cerevisiae/genetics
Chemicals
Fungal Proteins Oligonucleotide Probes Oligoribonucleotides RNA, Fungal RNA, Small Nuclear Ribonucleoproteins Endoribonucleases Ribonuclease H
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Xu Y
Department of Medical Genetics, University of Toronto, Ontario, Canada.
Petersen-Bjørn S
Friesen J D
References (33)
33 references, click to expand
  1. Protein binding sites are conserved in U1 small nuclear RNA from insects and mammals.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1217-20 PMID: 6187007
  2. The purification and properties of micrococcal nuclease.
    J Biol Chem. 1961 Nov;236:3014-9 PMID: 13860347
  3. U1 small nuclear ribonucleoprotein studied by in vitro assembly.
    J Cell Biol. 1983 Jun;96(6):1751-5 PMID: 6133878
  4. Purification of snRNPs U1, U2, U4, U5 and U6 with 2,2,7-trimethylguanosine-specific antibody and definition of their constituent proteins reacting with anti-Sm and anti-(U1)RNP antisera.
    EMBO J. 1983;2(7):1129-35 PMID: 6194992
  5. U2 RNA shares a structural domain with U1, U4, and U5 RNAs.
    EMBO J. 1982;1(10):1259-65 PMID: 6202507
  6. Yeast mRNA splicing in vitro.
    J Biol Chem. 1985 Nov 25;260(27):14780-92 PMID: 2997224
  7. Splicing of messenger RNA precursors.
    Annu Rev Biochem. 1986;55:1119-50 PMID: 2943217
  8. Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein.
    Cell. 1986 Aug 29;46(5):697-704 PMID: 2427202
  9. A subset of yeast snRNA's contains functional binding sites for the highly conserved Sm antigen.
    Science. 1987 Jan 16;235(4786):328-31 PMID: 2948278
  10. Differential nuclease sensitivity identifies tight contacts between yeast pre-mRNA and spliceosomes.
    EMBO J. 1986 Dec 20;5(13):3517-23 PMID: 2435546
  11. Fungal small nuclear ribonucleoproteins share properties with plant and vertebrate U-snRNPs.
    EMBO J. 1987 Feb;6(2):469-76 PMID: 2953599
  12. Primary structure of human nuclear ribonucleoprotein particle C proteins: conservation of sequence and domain structures in heterogeneous nuclear RNA, mRNA, and pre-rRNA-binding proteins.
    Mol Cell Biol. 1987 May;7(5):1731-9 PMID: 3110598
  13. An essential snRNA from S. cerevisiae has properties predicted for U4, including interaction with a U6-like snRNA.
    Cell. 1987 Aug 14;50(4):585-92 PMID: 2440583
  14. Identification of a yeast snRNP protein and detection of snRNP-snRNP interactions.
    Cell. 1987 Dec 24;51(6):1019-26 PMID: 2961458
  15. In vitro assembly of U1 snRNPs.
    EMBO J. 1987 Nov;6(11):3479-85 PMID: 2962858
  16. Spliceosome assembly in yeast.
    Genes Dev. 1987 Nov;1(9):1014-27 PMID: 2962902
  17. Reconstitution of the U1 small nuclear ribonucleoprotein particle.
    Mol Cell Biol. 1987 Nov;7(11):4030-7 PMID: 2963210
  18. The Mr 70,000 protein of the U1 small nuclear ribonucleoprotein particle binds to the 5' stem-loop of U1 RNA and interacts with Sm domain proteins.
    Proc Natl Acad Sci U S A. 1988 Feb;85(3):747-51 PMID: 2963336
  19. Spliceosomal RNA U6 is remarkably conserved from yeast to mammals.
    Nature. 1988 Jul 21;334(6179):213-8 PMID: 3041282
  20. Assembly of functional U1 and U2 human-amphibian hybrid snRNPs in Xenopus laevis oocytes.
    Science. 1988 Sep 9;241(4871):1328-31 PMID: 2970672
  21. Human U1 snRNP-specific C protein: complete cDNA and protein sequence and identification of a multigene family in mammals.
    Nucleic Acids Res. 1988 Sep 12;16(17):8307-21 PMID: 2971157
  22. Loop I of U1 small nuclear RNA is the only essential RNA sequence for binding of specific U1 small nuclear ribonucleoprotein particle proteins.
    Mol Cell Biol. 1988 Nov;8(11):4787-91 PMID: 2974920
  23. A binding consensus: RNA-protein interactions in splicing, snRNPs, and sex.
    Cell. 1989 Apr 7;57(1):1-3 PMID: 2522817
  24. A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein.
    Cell. 1989 Apr 7;57(1):89-101 PMID: 2467746
  25. In vitro reconstitution of snRNPs: a reconstituted U4/U6 snRNP participates in splicing complex formation.
    Genes Dev. 1989 Apr;3(4):479-87 PMID: 2524422
  26. 20S small nuclear ribonucleoprotein U5 shows a surprisingly complex protein composition.
    Proc Natl Acad Sci U S A. 1989 Aug;86(16):6038-42 PMID: 2527369
  27. The U1 RNA-binding site of the U1 small nuclear ribonucleoprotein (snRNP)-associated A protein suggests a similarity with U2 snRNPs.
    Mol Cell Biol. 1989 Jul;9(7):2975-82 PMID: 2528681
  28. PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle.
    Mol Cell Biol. 1989 Sep;9(9):3698-709 PMID: 2528686
  29. PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle.
    Mol Cell Biol. 1989 Sep;9(9):3710-9 PMID: 2528687
  30. Sequence-specific recognition of RNA hairpins by bacteriophage antiterminators requires a conserved arginine-rich motif.
    Cell. 1989 Oct 6;59(1):207-18 PMID: 2477156
  31. U5 small nuclear ribonucleoprotein: RNA structure analysis and ATP-dependent interaction with U4/U6.
    Mol Cell Biol. 1989 Aug;9(8):3350-9 PMID: 2552294
  32. U1 small nuclear ribonucleoprotein particle-specific proteins interact with the first and second stem-loops of U1 RNA, with the A protein binding directly to the RNA independently of the 70K and Sm proteins.
    Mol Cell Biol. 1989 Aug;9(8):3360-8 PMID: 2529425
  33. Structural organization of ribonucleoproteins containing small nuclear RNAs from HeLa cells. Proteins interact closely with a similar structural domain of U1, U2, U4 and U5 small nuclear RNAs.
    J Mol Biol. 1982 Dec 15;162(3):623-43 PMID: 6187926
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.3.1217-1225.1990
Published
1990-03-00
Pages
1217-25
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361002
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.10.3.1217-1225.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2