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PMID: 2156260 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Neoplastic transformation and tumorigenesis by the human protooncogene MYC.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 6 ·1990-03-00 ·Pages 2102-6

Ramsay GM, Moscovici G, Moscovici C, Bishop JM

Abstract

Damage to the protooncogene MYC has been implicated in the genesis of diverse human tumors, but the tumorigenic potential of the isolated gene has been disputed. Here we report the use of a retroviral vector to test the potency of human MYC for neoplastic transformation in avian cells. We found that sustained and abundant expression of MYC can transform both embryonic fibroblasts and hematopoietic cells and elicit granulocytic leukemias in chickens. Transformation by MYC is accompanied by changes in diverse aspects of cellular phenotype, including morphology, ability to grow in suspension, rate of proliferation, the structure of the cytoskeleton, and the composition of the extracellular matrix. Nevertheless, the biological potency of MYC is inherently constrained when compared to that of the retroviral oncogene v-myc. Our findings enlarge on previous descriptions of neoplastic transformation by MYC and sustain the view that ungoverned expression of the gene can contribute to the genesis of human tumors.

MeSH Terms
Actins/analysis Animals Avian Sarcoma Viruses/genetics Cell Division Cell Transformation, Neoplastic Cells, Cultured Chickens Fibroblasts/cytology Fibronectins/analysis Fluorescent Antibody Technique Genetic Vectors Humans Phenotype Protein-Tyrosine Kinases/genetics Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-myc Proto-Oncogenes Transfection
Chemicals
Actins Fibronectins Proto-Oncogene Proteins Proto-Oncogene Proteins c-myc Protein-Tyrosine Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ramsay G M
Department of Microbiology and Immunology, University of California, San Francisco 94143.
Moscovici G
Moscovici C
Bishop J M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.6.2102
Published
1990-03-00
Pages
2102-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53634
Subset
IM
Grants
NCI NIH HHS · CA 44338 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.6.2102 PMC Full Text View journal details
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