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PMID: 21572425 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

A universal code for RNA recognition by PUF proteins.

Nature chemical biology ·Vol. 7 ·No. 7 ·2011-05-15 ·Pages 425-7

Filipovska A, Razif MF, Nygård KK, Rackham O

Abstract

The design of proteins that can bind any RNA sequence of interest has many potential biological and medical applications. Here we have expanded the recognition of Pumilio and FBF homology protein (PUF) repeats beyond adenine, guanine and uracil and evolved them to specifically bind cytosine. These repeat sequences can be used to create PUF domains capable of selectively binding RNA targets of diverse sequence and structure.

MeSH Terms
Cytosine/metabolism Electrophoretic Mobility Shift Assay Humans Mutation Protein Engineering RNA/chemistry,genetics,metabolism RNA-Binding Proteins/chemistry,genetics,metabolism Repetitive Sequences, Amino Acid Saccharomyces cerevisiae/genetics,metabolism Two-Hybrid System Techniques
Chemicals
PUM1 protein, human RNA-Binding Proteins RNA Cytosine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Filipovska Aleksandra
Western Australian Institute for Medical Research and Centre for Medical Research, The University of Western Australia, Perth, Australia.
Razif Muhammad F M
Nygård Karoline K A
Rackham Oliver
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Article Info
Journal
Nature chemical biology
Abbr.
Nat Chem Biol
ISSN
1552-4469
DOI
10.1038/nchembio.577
Published
2011-05-15
Epub
2011-00-15
Pages
425-7
Language
English
Region
United States
NLM ID
101231976
Subset
IM
Corrections
CommentIn
PMID: 21802002
External Links
PubMed source DOI: 10.1038/nchembio.577 View journal details
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