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PMID: 2157284 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Binding of GAP to activated PDGF receptors.

Science (New York, N.Y.) ·Vol. 247 ·No. 4950 ·1990-03-30 ·Pages 1578-81

Kazlauskas A, Ellis C, Pawson T, Cooper JA

Abstract

The ras proto-oncogene products appear to relay intracellular signals via the Ras guanosine triphosphatase (GTPase) activator protein, GAP. In dog epithelial cells expressing human platelet-derived growth factor (PDGF) receptors, binding of PDGF caused approximately one-tenth of the total GAP molecules to complex with the receptor. Studies with mutant PDGF receptors showed that maximum association required both receptor kinase activity and phosphorylatable tyrosine residues at both the identified sites of receptor autophosphorylation.

MeSH Terms
Animals Cells, Cultured Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel GTP Phosphohydrolases/metabolism GTPase-Activating Proteins Humans Immunoblotting Oncogene Protein p21(ras)/metabolism Peptide Mapping Phosphopeptides/analysis Phosphorylation Platelet-Derived Growth Factor/metabolism Precipitin Tests Protein Kinases/analysis Proteins/metabolism Proto-Oncogene Mas Receptors, Cell Surface/metabolism Receptors, Platelet-Derived Growth Factor Tyrosine/metabolism ras GTPase-Activating Proteins
Chemicals
GTPase-Activating Proteins MAS1 protein, human Phosphopeptides Platelet-Derived Growth Factor Proteins Proto-Oncogene Mas Receptors, Cell Surface ras GTPase-Activating Proteins Tyrosine Protein Kinases Receptors, Platelet-Derived Growth Factor GTP Phosphohydrolases Oncogene Protein p21(ras)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kazlauskas A
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Ellis C
Pawson T
Cooper J A
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.2157284
Published
1990-03-30
Pages
1578-81
Language
English
Region
United States
NLM ID
0404511
Subset
IM
Grants
NCI NIH HHS · CA-28151 · United States
External Links
PubMed source DOI: 10.1126/science.2157284 View journal details
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