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PMID: 2160081 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Alkylation interference identifies essential DNA contacts for sequence-specific binding of the eukaryotic transcription factor C/EBP.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 10 ·1990-05-00 ·Pages 3992-6

Nye JA, Graves BJ

Abstract

Transcriptional regulators containing a "leucine zipper" and a flanking basic domain belong to a recently identified class of DNA-binding proteins. We have mapped the essential DNA contacts of one member of this group, the eukaryotic transcription factor C/EBP. Methylation and ethylation interference experiments detected major groove contacts over a full turn of the DNA helix on both an asymmetric and a symmetric C/EBP binding site. The contacts essential for C/EBP binding have two-fold symmetry yet differ significantly from the contacts of other dimeric DNA-binding proteins, including those bearing helix-turn-helix motifs and the type I restriction endonuclease EcoRI.

MeSH Terms
Alkylation Base Sequence Binding Sites CCAAT-Enhancer-Binding Proteins DNA, Viral/drug effects,metabolism DNA-Binding Proteins/metabolism Ethylnitrosourea/pharmacology Models, Molecular Molecular Sequence Data Nuclear Proteins/metabolism Nucleic Acid Conformation Promoter Regions, Genetic Repetitive Sequences, Nucleic Acid Restriction Mapping Sarcoma Viruses, Murine/genetics Transcription Factors/metabolism
Chemicals
CCAAT-Enhancer-Binding Proteins DNA, Viral DNA-Binding Proteins Nuclear Proteins Transcription Factors Ethylnitrosourea
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nye J A
Department of Cellular, Viral and Molecular Biology, University of Utah School of Medicine, Salt Lake City 84132.
Graves B J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.10.3992
Published
1990-05-00
Pages
3992-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54030
Subset
IM
Grants
NIGMS NIH HHS · R01 GM038663 · United States
NIGMS NIH HHS · GM38663 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.10.3992 PMC Full Text View journal details
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