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PMID: 2160435 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Expression of Salmonella typhimurium genes required for invasion is regulated by changes in DNA supercoiling.

Infection and immunity ·Vol. 58 ·No. 6 ·1990-06-00 ·Pages 1879-85

Galán JE, Curtiss R

Abstract

The ability to enter intestinal epithelial cells is an essential virulence factor of salmonellae. We have previously cloned a group of genes (invA, B, C, and D) that allow S. typhimurium to penetrate tissue culture cells (J. E. Galán and R. Curtiss III, Proc. Natl. Acad. Sci. USA 86:6383-6387, 1989). Transcriptional and translational cat and phoA fusions to invA (the proximal gene in the invABC operon) were constructed, and their expression was studied by measuring the levels of alkaline phosphatase or chloramphenicol acetyltransferase activity in mutants grown under different conditions. It was found that when strains containing the fusions were grown on media with high osmolarity, a condition known to increase DNA superhelicity, the level of invA transcription was approximately eightfold higher than that in strains grown on media with low osmolarity. The osmoinducibility of invA was independent of ompR, which controls the osmoinducibility of other genes. Strains grown in high-osmolarity media in the presence of subinhibitory concentrations of gyrase inhibitors (novobiocin or coumermycin A1), which reduce the level of DNA supercoiling, showed reduced expression of invA. Nevertheless, invA was poorly expressed in topA mutants of S. typhimurium, which have increased DNA superhelicity. In all cases, the differential expression of the invasion genes was correlated with the ability of S. typhimurium to penetrate tissue culture cells. These results taken together indicate that expression of S. typhimurium invasion genes is affected by changes in DNA supercoiling and suggest that this may represent a way in which this organism regulates the expression of these genes.

MeSH Terms
Animals Cell Line DNA Mutational Analysis DNA Topoisomerases, Type I/physiology DNA Topoisomerases, Type II/physiology DNA, Bacterial/genetics,ultrastructure DNA, Superhelical/genetics Dogs Epithelium/microbiology Gene Expression Regulation, Bacterial Genes, Regulator Osmolar Concentration Salmonella typhimurium/genetics,pathogenicity
Chemicals
DNA, Bacterial DNA, Superhelical DNA Topoisomerases, Type I DNA Topoisomerases, Type II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Galán J E
Department of Biology, Washington University, St. Louis, Missouri 63130.
Curtiss R
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.6.1879-1885.1990
Published
1990-06-00
Pages
1879-85
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258738
Subset
IM
Grants
NIAID NIH HHS · AI24533 · United States
External Links
PubMed source DOI: 10.1128/iai.58.6.1879-1885.1990 PMC Full Text View journal details
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