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PMID: 21627854 Published · epublish English Journal Article Research Support, N.I.H., Intramural Review

Microarrays, deep sequencing and the true measure of the transcriptome.

BMC biology ·Vol. 9 ·2011-05-31 ·Pages 34

Malone JH, Oliver B

Abstract

Microarrays first made the analysis of the transcriptome possible, and have produced much important information. Today, however, researchers are increasingly turning to direct high-throughput sequencing -- RNA-Seq -- which has considerable advantages for examining transcriptome fine structure -- for example in the detection of allele-specific expression and splice junctions. In this article, we discuss the relative merits of the two techniques, the inherent biases in each, and whether all of the vast body of array work needs to be revisited using the newer technology. We conclude that microarrays remain useful and accurate tools for measuring expression levels, and RNA-Seq complements and extends microarray measurements.

MeSH Terms
Animals Drosophila/genetics Gene Expression Profiling High-Throughput Nucleotide Sequencing Humans Oligonucleotide Array Sequence Analysis/methods Sequence Analysis, RNA/methods
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Malone John H
Laboratory of Cellular and Developmental Biology, National Institute of Digestive, Diabetes, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA. [email protected]
Oliver Brian
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Article Info
Journal
BMC biology
Abbr.
BMC Biol
ISSN
1741-7007
DOI
10.1186/1741-7007-9-34
Email
[email protected]
Published
2011-05-31
Epub
2011-00-31
Pages
34
Language
English
Region
England
NLM ID
101190720
PMCID
PMC3104486
Subset
IM
Grants
Intramural NIH HHS · ZIA DK015600-15 · United States
External Links
PubMed source DOI: 10.1186/1741-7007-9-34 PMC Full Text View journal details
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