Sharper low-power STED nanoscopy by time gating.

Nature methods ·Vol. 8 ·No. 7 ·2011-06-05 ·Pages 571-3

Vicidomini G, Moneron G, Han KY, Westphal V, Ta H, Reuss M, Engelhardt J, Eggeling C, Hell SW

Abstract

Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells.

MeSH Terms

Animals Cell Line Cell Membrane/chemistry Light Lipids/analysis,chemistry Macropodidae Microscopy, Fluorescence/methods Time Factors

Chemicals

Lipids

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Vicidomini Giuseppe

Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Göttingen, Germany.

Moneron Gael

Han Kyu Y

Westphal Volker

Ta Haisen

Reuss Matthias

Engelhardt Johann

Eggeling Christian

Hell Stefan W

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Article Info

Journal

Nature methods

Abbr.

Nat Methods

ISSN

1548-7105

DOI

10.1038/nmeth.1624

Published

2011-06-05

Epub

2011-00-05

Pages

571-3

Language

English

Region

United States

NLM ID

101215604

Subset

External Links

PubMed source DOI: 10.1038/nmeth.1624 View journal details

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