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PMID: 21655959 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Peroxisomal localisation of the final steps of the mevalonic acid pathway in planta.

Planta ·Vol. 234 ·No. 5 ·2011-11-00 ·Pages 903-14

Simkin AJ, Guirimand G, Papon N, Courdavault V, Thabet I, Ginis O, Bouzid S, Giglioli-Guivarc'h N, Clastre M

Abstract

In plants, the mevalonic acid (MVA) pathway provides precursors for the formation of triterpenes, sesquiterpenes, phytosterols and primary metabolites important for cell integrity. Here, we have cloned the cDNA encoding enzymes catalysing the final three steps of the MVA pathway from Madagascar periwinkle (Catharanthus roseus), mevalonate kinase (MVK), 5-phosphomevalonate kinase (PMK) and mevalonate 5-diphosphate decarboxylase (MVD). These cDNA were shown to functionally complement MVA pathway deletion mutants in the yeast Saccharomyces cerevisiae. Transient transformations of C. roseus cells with yellow fluorescent protein (YFP)-fused constructs reveal that PMK and MVD are localised to the peroxisomes, while MVK was cytosolic. These compartmentalisation results were confirmed using the Arabidopsis thaliana MVK, PMK and MVD sequences fused to YFP. Based on these observations and the arguments raised here we conclude that the final steps of the plant MVA pathway are localised to the peroxisome.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics,metabolism Catharanthus/enzymology,genetics Cloning, Molecular Cytosol/metabolism DNA, Complementary/genetics,metabolism Gene Expression Regulation, Plant Genetic Complementation Test Luminescent Proteins/metabolism Mevalonic Acid/analogs & derivatives,metabolism Molecular Sequence Data Peroxisomes/metabolism Phosphotransferases (Alcohol Group Acceptor)/genetics,metabolism Phosphotransferases (Phosphate Group Acceptor)/genetics,metabolism RNA, Plant/genetics Saccharomyces cerevisiae/genetics,metabolism Transformation, Genetic
Chemicals
DNA, Complementary Luminescent Proteins RNA, Plant 5-diphosphomevalonic acid Phosphotransferases (Alcohol Group Acceptor) mevalonate kinase Phosphotransferases (Phosphate Group Acceptor) phosphomevalonate kinase Mevalonic Acid
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Simkin Andrew J
EA 2106, Biomolécules et Biotechnologies Végétales, Université François-Rabelais de Tours, 31 Avenue Monge, 37200, Tours, France. [email protected]
Guirimand Grégory
Papon Nicolas
Courdavault Vincent
Thabet Insaf
Ginis Olivia
Bouzid Sadok
Giglioli-Guivarc'h Nathalie
Clastre Marc
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Article Info
Journal
Planta
Abbr.
Planta
ISSN
1432-2048
DOI
10.1007/s00425-011-1444-6
Email
[email protected]
Published
2011-11-00
Epub
2011-00-08
Pages
903-14
Language
English
Region
Germany
NLM ID
1250576
Subset
IM
External Links
PubMed source DOI: 10.1007/s00425-011-1444-6 View journal details
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