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PMID: 21660700 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Homologous recombination assay for interstrand cross-link repair.

Methods in molecular biology (Clifton, N.J.) ·Vol. 745 ·2011-00-00 ·Pages 283-91

Nakanishi K, Cavallo F, Brunet E, Jasin M

Abstract

DNA interstrand cross-links (ICLs) covalently link both strands of the DNA duplex, impeding cellular processes like DNA replication. Homologous recombination (HR) is considered to be a major pathway for the repair of ICLs in mammalian cells as mutants for HR components are highly sensitive to DNA-damaging agents that cause ICLs. This chapter describes GFP assays to measure HR following site-specific ICL formation with psoralen through DNA triplex technology. This approach can be used to determine the genetic requirements for ICL-induced HR in relation to those involved in HR repair of other DNA lesions such as double-strand breaks.

MeSH Terms
Cell Line, Tumor DNA Repair/genetics Flow Cytometry Humans Oligonucleotides/genetics Plasmids/genetics Recombination, Genetic/genetics
Chemicals
Oligonucleotides
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nakanishi Koji
Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA. [email protected]
Cavallo Francesca
Brunet Erika
Jasin Maria
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Article Info
Journal
Methods in molecular biology (Clifton, N.J.)
Abbr.
Methods Mol Biol
ISSN
1940-6029
DOI
10.1007/978-1-61779-129-1_16
Email
[email protected]
Published
2011-00-00
Pages
283-91
Language
English
Region
United States
NLM ID
9214969
PMCID
PMC3261721
Subset
IM
Grants
NCI NIH HHS · P01 CA094060-10 · United States
NCI NIH HHS · P01CA94060 · United States
NIGMS NIH HHS · R01 GM054668 · United States
NCI NIH HHS · P01 CA094060 · United States
NIGMS NIH HHS · R01GM54668 · United States
NIGMS NIH HHS · R01 GM054668-12 · United States
NIGMS NIH HHS · R01 GM054668-12S1 · United States
External Links
PubMed source DOI: 10.1007/978-1-61779-129-1_16 PMC Full Text
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