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PMID: 21673079 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The MYB80 transcription factor is required for pollen development and the regulation of tapetal programmed cell death in Arabidopsis thaliana.

The Plant cell ·Vol. 23 ·No. 6 ·2011-06-00 ·Pages 2209-24

Phan HA, Iacuone S, Li SF, Parish RW

Abstract

Arabidopsis thaliana MYB80 (formerly MYB103) is expressed in the tapetum and microspores between anther developmental stages 6 and 10. MYB80 encodes a MYB transcription factor that is essential for tapetal and pollen development. Using microarray analysis of anther mRNA, we identified 404 genes differentially expressed in the myb80 mutant. Employing the glucocorticoid receptor system, the expression of 79 genes was changed when MYB80 function was restored in the myb80 mutant following induction by dexamethasone. Thirty-two genes were analyzed using chromatin immunoprecipitation, and three were identified as direct targets of MYB80. The genes encode a glyoxal oxidase (GLOX1), a pectin methylesterase (VANGUARD1), and an A1 aspartic protease (UNDEAD). All three genes are expressed in the tapetum and microspores. Electrophoretic mobility shift assays confirmed that MYB80 binds to all three target promoters, with the preferential binding site containing the CCAACC motif. TUNEL assays showed that when UNDEAD expression was silenced using small interfering RNA, premature tapetal and pollen programmed cell death occurred, resembling the myb80 mutant phenotype. UNDEAD possesses a mitochondrial targeting signal and may hydrolyze an apoptosis-inducing protein(s) in mitochondria. The timing of tapetal programmed cell death is critical for pollen development, and the MYB80/UNDEAD system may regulate that timing.

MeSH Terms
Arabidopsis/anatomy & histology,genetics,growth & development,metabolism Arabidopsis Proteins/genetics,metabolism Base Sequence Carboxylic Ester Hydrolases/genetics,metabolism Cell Death/physiology Chromatin Immunoprecipitation Flowers/cytology,physiology Gene Expression Profiling Gene Expression Regulation, Plant Microarray Analysis Molecular Sequence Data Plants, Genetically Modified Pollen/cytology,growth & development Promoter Regions, Genetic RNA, Small Interfering/metabolism Recombinant Fusion Proteins/genetics,metabolism Transcription Factors/genetics,metabolism
Chemicals
Arabidopsis Proteins MYB103 protein, Arabidopsis RNA, Small Interfering Recombinant Fusion Proteins Transcription Factors Carboxylic Ester Hydrolases VGD1 protein, Arabidopsis
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Phan Huy Anh
Botany Department, La Trobe University, Melbourne, Victoria 3086, Australia.
Iacuone Sylvana
Li Song F
Parish Roger W
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.110.082651
Published
2011-06-00
Epub
2011-00-14
Pages
2209-24
Language
English
Region
England
NLM ID
9208688
PMCID
PMC3160043
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.110.082651 PMC Full Text View journal details
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