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PMID: 2168387 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Efficient incorporation of galactose into lipopolysaccharide by Escherichia coli K-12 strains with polar galE mutations.

Journal of bacteriology ·Vol. 172 ·No. 9 ·1990-09-00 ·Pages 5511-3

Schnaitman CA, Austin EA

Abstract

Efficient incorporation of exogenous galactose into lipopolysaccharide was observed in a strain with a galE::Tn10 insertion and a strain with a deletion of galOPE. No incorporation was observed in a strain with a longer (galETK) deletion, indicating that incorporation into the galE mutants was due to a low level of expression of galTK from internal or fortuitous promoters. These galE mutants provided a convenient and specific way to radiolabel lipopolysaccharide.

MeSH Terms
Cell Membrane/metabolism Chromosome Deletion DNA Transposable Elements Escherichia coli/genetics,metabolism Galactose/metabolism Kinetics Lipopolysaccharides/biosynthesis Mutation Operon
Chemicals
DNA Transposable Elements Lipopolysaccharides Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schnaitman C A
Department of Microbiology, University of Virginia Medical School, Charlottesville 22908.
Austin E A
References (14)
14 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.9.5511-5513.1990
Published
1990-09-00
Pages
5511-3
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213224
Subset
IM
Grants
NIGMS NIH HHS · GM-39087 · United States
External Links
PubMed source DOI: 10.1128/jb.172.9.5511-5513.1990 PMC Full Text View journal details
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