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PMID: 2169461 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genomic variation of Trypanosoma cruzi: involvement of multicopy genes.

Infection and immunity ·Vol. 58 ·No. 10 ·1990-10-00 ·Pages 3217-24

Wagner W, So M

Abstract

By using improved pulsed field gel conditions, the karyotypes of several strains of the protozoan parasite Trypanosoma cruzi were analyzed and compared with those of Leishmania major and two other members of the genus Trypanosoma. There was no difference in chromosome migration patterns between different life cycle stages of the T. cruzi strains analyzed. However, the sizes and numbers of chromosomal bands varied considerably among T. cruzi strains. This karyotype variation among T. cruzi strains was analyzed further at the chromosomal level by using multicopy genes as probes in Southern hybridizations. The chromosomal location of the genes encoding alpha- and beta-tubulin, ubiquitin, rRNA, spliced leader RNA, and an 85-kilodalton protein remained stable during developmental conversion of the parasite. The sizes and numbers of chromosomes containing these sequences varied among the different strains analyzed, implying multiple rearrangements of these genes during evolution of the parasites. During continuous in vitro cultivation of T. cruzi Y, the chromosomal location of the spliced leader gene shifted spontaneously. The spliced leader gene encodes a 35-nucleotide RNA that is spliced in trans from a 105-nucleotide donor RNA onto all mRNAs in T. cruzi. The spliced leader sequences changed in their physical location in both the cloned and uncloned Y strains. Associated with the complex changes was an increase in the infectivity of the rearranged variant for tissue culture cells. Our results indicate that the spliced leader gene clusters in T. cruzi undergo high-frequency genomic rearrangements.

MeSH Terms
Animals Base Sequence Blotting, Southern Cells, Cultured Chromosomes/analysis Electrophoresis, Agar Gel Genetic Variation Karyotyping Leishmania tropica/genetics Molecular Sequence Data Multigene Family Nucleic Acid Hybridization Protozoan Proteins/genetics RNA Probes RNA, Messenger/genetics RNA, Ribosomal/genetics Selection, Genetic Trypanosoma cruzi/genetics Tubulin/genetics Ubiquitins/genetics
Chemicals
Protozoan Proteins RNA Probes RNA, Messenger RNA, Ribosomal Tubulin Ubiquitins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wagner W
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
So M
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.10.3217-3224.1990
Published
1990-10-00
Pages
3217-24
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313642
Subset
IM
Grants
NIAID NIH HHS · AI 22983 · United States
External Links
PubMed source DOI: 10.1128/iai.58.10.3217-3224.1990 PMC Full Text View journal details
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