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PMID: 2170327 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

General organization of the conjugal transfer genes of the IncW plasmid R388 and interactions between R388 and IncN and IncP plasmids.

Journal of bacteriology ·Vol. 172 ·No. 10 ·1990-10-00 ·Pages 5795-802

Bolland S, Llosa M, Avila P, de la Cruz F

Abstract

The complete conjugal transfer gene region of the IncW plasmid R388 has been cloned in multicopy vector plasmids and mapped to a contiguous 14.9-kilobase segment by insertion mutagenesis. The fertility of the cloned region could still be inhibited by a coresident IncP plasmid. The transfer region has been dissected into two regions, one involved in pilus synthesis and assembly (PILW), and the other involved in conjugal DNA metabolism (MOBW). They have been separately cloned. PILW also contains the genes involved in entry exclusion. MOBW contains oriT and the gene products required for efficient mobilization by PILW. MOBW plasmids could also be mobilized efficiently by PILN, the specific pilus of the IncN plasmid pCU1, but not by PILP, the specific pilus of the IncP plasmid RP1.

MeSH Terms
Cloning, Molecular/methods Conjugation, Genetic DNA Transposable Elements Escherichia coli/genetics Genes, Bacterial Mutation Plasmids Restriction Mapping Transformation, Bacterial
Chemicals
DNA Transposable Elements
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bolland S
Departamento de Biología Molecular, Universidad de Cantabria, Santander, Spain.
Llosa M
Avila P
de la Cruz F
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.10.5795-5802.1990
Published
1990-10-00
Pages
5795-802
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC526897
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.10.5795-5802.1990 PMC Full Text View journal details
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