Home LiteratureArticle Details

PMID: 2171967 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Nuclear formation in a Drosophila cell-free system.

Experimental cell research ·Vol. 191 ·No. 1 ·1990-11-00 ·Pages 64-70

Berrios M, Avilion AA

Abstract

A cell-free preparation obtained from 0- to 5-h-old Drosophila melanogaster embryos induces chromatin decondensation and nuclear formation from demembranated Xenopus sperm. Newly formed nuclei have a peripheral lamina, a double membrane, and structures resembling pore complexes. Indirect immunofluorescence analyses demonstrate the association of Drosophila lamins and DNA topoisomerase II with newly assembled nuclei.

MeSH Terms

Animals Antibodies, Monoclonal Cell Nucleus/enzymology,metabolism,ultrastructure Cell-Free System Chromatin DNA Topoisomerases, Type II/metabolism Drosophila melanogaster Embryo, Nonmammalian Fluorescent Antibody Technique In Vitro Techniques Lamins Male Nuclear Envelope/metabolism,ultrastructure Nuclear Proteins/metabolism Spermatozoa/ultrastructure Xenopus

Chemicals

Antibodies, Monoclonal Chromatin Lamins Nuclear Proteins DNA Topoisomerases, Type II

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Berrios M

Department of Pharmacological Sciences, School of Medicine, State University of New York, Stony Brook 11794-8651.

Avilion A A

Article Info

Journal

Experimental cell research

Abbr.

Exp Cell Res

ISSN

0014-4827

DOI

10.1016/0014-4827(90)90036-a

Published

1990-11-00

Pages

64-70

Language

English

Region

United States

NLM ID

0373226

Subset

External Links

PubMed source DOI: 10.1016/0014-4827(90)90036-a View journal details

Analysis Services

Nuclear formation in a Drosophila cell-free system.

Contact

WeChat Official Account