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PMID: 2172985 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Regulation of the fibroblast growth factor receptor in early Xenopus embryos.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 21 ·1990-11-00 ·Pages 8365-9

Musci TJ, Amaya E, Kirschner MW

Abstract

Recent evidence suggests that fibroblast growth factor (FGF) is a primary mesoderm inducer in Xenopus development. We have isolated a full-length cDNA clone for the Xenopus FGF receptor. Like other FGF receptors, the Xenopus homolog is a membrane-spanning protein with a split intracellular tyrosine kinase domain. The Xenopus FGF receptor mRNA is present as a maternal message whose levels are constant through early development. There is no specific regional localization of the transcript by analysis of FGF receptor mRNA levels in microdissected embryonic tissue. In isolated animal-pole blastomeres, FGF receptor mRNA declines over 16 hr in culture and this loss can be prevented by incubation with FGF or activin. Despite the presence of the FGF receptor mRNA in the oocyte, oocytes in culture do not respond to added FGF. However, injection of exogenous Xenopus FGF receptor transcripts into oocytes does generate a functional response to FGF. Our data suggest that posttranscriptional response to FGF. Our data suggest that posttranscriptional mechanisms regulate the FGF receptor in the oocyte and early embryo and further suggest that mesoderm-inducing factors influence receptor mRNA levels during the time of early tissue formation.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Calcium/metabolism Cloning, Molecular DNA/genetics Embryo, Nonmammalian/physiology Female Fibroblast Growth Factor 2/pharmacology Fibroblast Growth Factors/metabolism Molecular Sequence Data Oligonucleotide Probes Oocytes/drug effects,physiology RNA, Messenger/genetics Receptors, Cell Surface/genetics Receptors, Fibroblast Growth Factor Sequence Homology, Nucleic Acid Xenopus laevis/embryology
Chemicals
Oligonucleotide Probes RNA, Messenger Receptors, Cell Surface Receptors, Fibroblast Growth Factor Fibroblast Growth Factor 2 Fibroblast Growth Factors DNA Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Musci T J
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Amaya E
Kirschner M W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.21.8365
Published
1990-11-00
Pages
8365-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54956
Subset
IM
Databases
GENBANK
M37201, M61687
External Links
PubMed source DOI: 10.1073/pnas.87.21.8365 PMC Full Text View journal details
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