Recognition of the beta-subunit of human chorionic gonadotropin and sub-determinants by target tissue receptors.

The Biochemical journal ·Vol. 176 ·No. 2 ·1978-11-15 ·Pages 599-602

Ramakrishnan S, Das C, Talwar GP

Abstract

The beta-subunit of human chorionic gonadotropin, purified immunochemically to eliminate undissociated human chorionic gonadotropin, induced testosterone production by mouse Leydig cells at concentrations 400-fold higher than human chorionic gonadotropin. Steroidogenesis was also stimulated by a synthetic fragment of the beta-subunit of human chorionic gonadotropin conforming to the peptide sequence residues 39--71, whereas peptide sequence residues 39--56 and three C-terminal fragments (residues 115--145, 111--145 and 101--145) failed to cause steroidogenesis. These studies suggest the presence in the beta-subunit of human chorionic gonadotropin of determinants recognized by the tissue receptors, a part of these determinants residing between amino acid residues 57--71.

MeSH Terms

Animals Chorionic Gonadotropin/isolation & purification,pharmacology In Vitro Techniques Leydig Cells/drug effects,metabolism Male Mice Peptide Fragments/pharmacology Receptors, Cell Surface/drug effects Testosterone/biosynthesis

Chemicals

Chorionic Gonadotropin Peptide Fragments Receptors, Cell Surface Testosterone

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Ramakrishnan S

Das C

Talwar G P

References (25)

25 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1760599

Published

1978-11-15

Pages

599-602

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1186271

Subset

External Links

PubMed source DOI: 10.1042/bj1760599 PMC Full Text View journal details

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Recognition of the beta-subunit of human chorionic gonadotropin and sub-determinants by target tissue receptors.

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