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PMID: 2174353 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Regulation of the segmentation gene fushi tarazu has been functionally conserved in Drosophila.

The EMBO journal ·Vol. 9 ·No. 12 ·1990-12-00 ·Pages 3957-66

Maier D, Preiss A, Powell JR

Abstract

An evolutionary approach was applied to identify elements involved in the regulation of the segmentation gene fushi tarazu (ftz) by comparing the Drosophila melanogaster ftz gene with its Drosophila hydei homologue. The overall organization of the ftz gene is very similar in both species. Surprisingly, ftz proved to be inverted in the ANT-C of D. hydei with respect to D. melanogaster. Strong homologies extend over the entire 6 kb of the ftz upstream region with the best match in the 'upstream element'. We identified several highly conserved boxes embedded in unrelated sequences that correspond extremely well to two germ layer specific enhancers in the upstream element. Transformation experiments revealed that D. hydei ftz gene products can restore D. melanogaster ftz function and, furthermore, that trans-acting factors from D. melanogaster recognize and control D. hydei ftz regulatory elements. These findings indicate a conservation of the entire regulatory network among segmentation genes for several millions of years during the evolution of Drosophila.

Related Genes
ftz
MeSH Terms
Animals Base Sequence DNA Transposable Elements Drosophila/genetics Drosophila melanogaster/genetics Gene Expression Regulation Genes, Regulator Molecular Sequence Data Restriction Mapping Sequence Homology, Nucleic Acid Software
Chemicals
DNA Transposable Elements
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Maier D
Populationsgenetik, Biologie II, Universität Tübingen, FRG.
Preiss A
Powell J R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-12-00
Pages
3957-66
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552166
Subset
IM
Databases
GENBANK
X56038
External Links
PubMed source PMC Full Text View journal details
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