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PMID: 2176471 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Relationship between phosphoinositide kinase activities and protein tyrosine phosphorylation in plasma membranes from A431 cells.

The Biochemical journal ·Vol. 272 ·No. 3 ·1990-12-15 ·Pages 665-70

Payrastre B, Plantavid M, Breton M, Chambaz E, Chap H

Abstract

Production of PtdIns(4)P and PtdIns(4,5)P2 by plasma-membrane preparations from A431 cells was selectively stimulated in a dose-dependent manner by epidermal growth factor (EGF) in the presence of Na3VO4. Na3VO4 itself mimicked this effect, which was overcome after treatment by a specific phosphotyrosyl phosphatase isolated from A431 cells. PtdIns and PtdIns(4)P kinase activities were present in phosphotyrosyl-proteins isolated from EGF- and/or Na3VO4-stimulated A431 cells by immunoaffinity using an anti-phosphotyrosine antibody. These data suggest for the first time an EGF-dependent regulation of PtdIns 4-kinase and PtdIns(4)P 5-kinase activities by a mechanism involving a tyrosine-phosphorylation process.

MeSH Terms
1-Phosphatidylinositol 4-Kinase Adenosine Triphosphate/metabolism Carcinoma, Squamous Cell Cell Line Chromatography, High Pressure Liquid Humans Kinetics Membrane Proteins/isolation & purification,metabolism Molecular Weight Neoplasm Proteins/metabolism Phosphatidylinositols/isolation & purification,metabolism Phosphoprotein Phosphatases/metabolism Phosphoproteins/isolation & purification Phosphorus Radioisotopes Phosphorylation Phosphotransferases/metabolism Protein Tyrosine Phosphatases Protein-Tyrosine Kinases/metabolism
Chemicals
Membrane Proteins Neoplasm Proteins Phosphatidylinositols Phosphoproteins Phosphorus Radioisotopes Adenosine Triphosphate Phosphotransferases 1-Phosphatidylinositol 4-Kinase Protein-Tyrosine Kinases Phosphoprotein Phosphatases Protein Tyrosine Phosphatases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Payrastre B
INSERM U326, Hôpital Purpan, Toulouse, France.
Plantavid M
Breton M
Chambaz E
Chap H
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32 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2720665
Published
1990-12-15
Pages
665-70
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1149760
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2720665 PMC Full Text View journal details
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