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PMID: 21769440 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Neutrophilic inflammatory response and oxidative stress in premenopausal women chronically exposed to indoor air pollution from biomass burning.

Inflammation ·Vol. 35 ·No. 2 ·2012-04-00 ·Pages 671-83

Banerjee A, Mondal NK, Das D, Ray MR

Abstract

The possibility of inflammation and neutrophil activation in response to indoor air pollution (IAP) from biomass fuel use has been investigated. For this, 142 premenopausal, never-smoking women (median age, 34 years) who cook exclusively with biomass (wood, dung, crop wastes) and 126 age-matched control women who cook with cleaner fuel liquefied petroleum gas (LPG) were enrolled. The neutrophil count in blood and sputum was significantly higher (p < 0.05) in biomass users than the control group. Flow cytometric analysis revealed marked increase in the surface expression of CD35 (complement receptor-1), CD16 (F(C)γ receptor III), and β(2) Mac-1 integrin (CD11b/CD18) on circulating neutrophils of biomass users. Besides, enzyme-linked immunosorbent assay showed that they had 72%, 67%, and 54% higher plasma levels of the proinflammatory cytokines tumor necrosis factor-alpha, interleukin-6, and interleukin-12, respectively, and doubled neutrophil chemoattractant interleukin-8. Immunocytochemical study revealed significantly higher percentage of airway neutrophils expressing inducible nitric oxide synthase, while the serum level of nitric oxide was doubled in women who cooked with biomass. Spectrophotometric analysis documented higher myeloperoxidase activity in circulating neutrophils of biomass users, suggesting neutrophil activation. Flow cytometry showed excess generation of reactive oxygen species (ROS) by leukocytes of biomass-using women, whereas their erythrocytes contained a depleted level of antioxidant enzyme superoxide dismutase (SOD). Indoor air of biomass-using households had two to four times more particulate matter with diameters of <10 μm (PM(10)) and <2.5 μm (PM(2.5)) as measured by real-time laser photometer. After controlling potential confounders, rise in proinflammatory mediators among biomass users were positively associated with PM(10) and PM(2.5) in indoor air, suggesting a close relationship between IAP and neutrophil activation. Besides, the levels of neutrophil activation and inflammation markers were positively associated with generation of ROS and negatively with SOD, indicating a role of oxidative stress in mediating neutrophilic inflammatory response following chronic inhalation of biomass smoke.

MeSH Terms
Adult Air Pollution, Indoor/adverse effects Biofuels/adverse effects CD11b Antigen/metabolism CD18 Antigens/metabolism Female Humans Inflammation/immunology Interleukins/blood Leukocyte Count Neutrophil Activation Neutrophils/immunology Nitric Oxide/blood Nitric Oxide Synthase Type II/metabolism Oxidative Stress Particulate Matter/analysis,metabolism Peroxidase/biosynthesis Premenopause Reactive Oxygen Species/metabolism Receptors, Complement 3b/metabolism Receptors, IgG/metabolism Smoke/adverse effects Superoxide Dismutase/metabolism Tumor Necrosis Factor-alpha/blood Young Adult
Chemicals
Biofuels CD11b Antigen CD18 Antigens Interleukins Particulate Matter Reactive Oxygen Species Receptors, Complement 3b Receptors, IgG Smoke Tumor Necrosis Factor-alpha Nitric Oxide Peroxidase Nitric Oxide Synthase Type II Superoxide Dismutase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Banerjee Anirban
Department of Experimental Hematology, Chittaranjan National Cancer Institute, 37, S. P. Mukherjee Road, Kolkata, 700 026, India.
Mondal Nandan Kumar
Das Debangshu
Ray Manas Ranjan
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Article Info
Journal
Inflammation
Abbr.
Inflammation
ISSN
1573-2576
DOI
10.1007/s10753-011-9360-2
Published
2012-04-00
Pages
671-83
Language
English
Region
United States
NLM ID
7600105
Subset
IM
External Links
PubMed source DOI: 10.1007/s10753-011-9360-2 View journal details
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