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PMID: 21772271 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Arabidopsis nitrate reductase activity is stimulated by the E3 SUMO ligase AtSIZ1.

Nature communications ·Vol. 2 ·2011-07-19 ·Pages 400

Park BS, Song JT, Seo HS

Abstract

Small ubiquitin-related modifier (SUMO) is a small polypeptide that modulates protein activity and regulates hormone signalling, abiotic and biotic responses in plants. Here we show that AtSIZ regulates nitrogen assimilation in Arabidopsis through its E3 SUMO ligase function. Dwarf plants of siz1-2 flower early, show abnormal seed development and have high salicylic acid content and enhanced resistance to bacterial pathogens. These mutant phenotypes are reverted to wild-type phenotypes by exogenous ammonium but not by nitrate, phosphate or potassium. Decreased nitrate reductase activity in siz1-2 plants resulted in low nitrogen concentrations, low nitric oxide production and high nitrate content in comparison with wild-type plants. The nitrate reductases, NIA1 and NIA2, are sumoylated by AtSIZ1, which dramatically increases their activity. Both sumoylated and non-sumoylated NIA1 and NIA2 can form dimers. Our results indicate that AtSIZ1 positively controls nitrogen assimilation by promoting sumoylation of NRs in Arabidopsis.

MeSH Terms
Arabidopsis/enzymology,immunology,metabolism,microbiology Arabidopsis Proteins/genetics,metabolism Colony Count, Microbial Ligases/genetics,metabolism Mutation/genetics Nitrate Reductase/chemistry,metabolism Nitrates/analysis Nitric Oxide/biosynthesis Protein Multimerization Pseudomonas syringae/immunology Quaternary Ammonium Compounds/pharmacology Salicylic Acid/analysis Seeds/chemistry Small Ubiquitin-Related Modifier Proteins/metabolism Sumoylation Ubiquitin-Protein Ligases/metabolism
Chemicals
Arabidopsis Proteins Nitrates Quaternary Ammonium Compounds Small Ubiquitin-Related Modifier Proteins Nitric Oxide Nitrate Reductase Ubiquitin-Protein Ligases Ligases SIZ1 protein, Arabidopsis Salicylic Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Park Bong Soo
Department of Plant Science, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea.
Song Jong Tae
Seo Hak Soo
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Article Info
Journal
Nature communications
Abbr.
Nat Commun
ISSN
2041-1723
DOI
10.1038/ncomms1408
Published
2011-07-19
Epub
2011-00-19
Pages
400
Language
English
Region
England
NLM ID
101528555
PMCID
PMC3160146
Subset
IM
External Links
PubMed source DOI: 10.1038/ncomms1408 PMC Full Text View journal details
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