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PMID: 21804566 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

14-3-3 proteins act as intracellular receptors for rice Hd3a florigen.

Nature ·Vol. 476 ·No. 7360 ·2011-07-31 ·Pages 332-5

Taoka K, Ohki I, Tsuji H, Furuita K, Hayashi K, Yanase T, Yamaguchi M, Nakashima C, Purwestri YA, Tamaki S, Ogaki Y, Shimada C, Nakagawa A, Kojima C, Shimamoto K

Abstract

'Florigen' was proposed 75 years ago to be synthesized in the leaf and transported to the shoot apex, where it induces flowering. Only recently have genetic and biochemical studies established that florigen is encoded by FLOWERING LOCUS T (FT), a gene that is universally conserved in higher plants. Nonetheless, the exact function of florigen during floral induction remains poorly understood and receptors for florigen have not been identified. Here we show that the rice FT homologue Hd3a interacts with 14-3-3 proteins in the apical cells of shoots, yielding a complex that translocates to the nucleus and binds to the Oryza sativa (Os)FD1 transcription factor, a rice homologue of Arabidopsis thaliana FD. The resultant ternary 'florigen activation complex' (FAC) induces transcription of OsMADS15, a homologue of A. thaliana APETALA1 (AP1), which leads to flowering. We have determined the 2.4 Å crystal structure of rice FAC, which provides a mechanistic basis for florigen function in flowering. Our results indicate that 14-3-3 proteins act as intracellular receptors for florigen in shoot apical cells, and offer new approaches to manipulate flowering in various crops and trees.

MeSH Terms
14-3-3 Proteins/metabolism Arabidopsis Proteins/chemistry Calcium-Binding Proteins/chemistry Cell Nucleus/metabolism Flowers/growth & development,metabolism Gene Expression Regulation, Plant MADS Domain Proteins/chemistry Models, Molecular Multiprotein Complexes/chemistry,metabolism Oryza/genetics,growth & development,metabolism Plant Proteins/chemistry,genetics,metabolism Plant Shoots/cytology Protein Binding Sequence Homology, Amino Acid Transcription Factors/chemistry Two-Hybrid System Techniques
Chemicals
14-3-3 Proteins AP1 protein, Arabidopsis Arabidopsis Proteins Calcium-Binding Proteins FD protein, Arabidopsis FT protein, Arabidopsis GF14 protein, Arabidopsis MADS Domain Proteins Multiprotein Complexes Plant Proteins Transcription Factors
Authors & Affiliations
15 authors, click to expand affiliations / ORCID
Taoka Ken-ichiro
Laboratory of Plant Molecular Genetics, Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
Ohki Izuru
Tsuji Hiroyuki
Furuita Kyoko
Hayashi Kokoro
Yanase Tomoko
Yamaguchi Midori
Nakashima Chika
Purwestri Yekti Asih
Tamaki Shojiro
Ogaki Yuka
Shimada Chihiro
Nakagawa Atsushi
Kojima Chojiro
Shimamoto Ko
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Article Info
Journal
Nature
Abbr.
Nature
ISSN
1476-4687
DOI
10.1038/nature10272
Published
2011-07-31
Epub
2011-00-31
Pages
332-5
Language
English
Region
England
NLM ID
0410462
Subset
IM
Databases
PDB
3AXY
External Links
PubMed source DOI: 10.1038/nature10272 View journal details
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