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PMID: 2181156 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mutational analysis of the conserved cysteine-rich region of the human immunodeficiency virus type 1 Tat protein.

Journal of virology ·Vol. 64 ·No. 4 ·1990-04-00 ·Pages 1864-8

Rice AP, Carlotti F

Abstract

The Tat transactivator protein of human immunodeficiency virus type 1 contains a highly conserved cysteine-rich region, containing seven cysteines from residues 22 through 37. To investigate the importance of noncysteine residues in this region of the Tat protein, we have carried out a mutational analysis, in most cases substituting a single alanine for the wild-type noncysteine residue. Alanine substitution of residue 23, 24, 46, or 47 had no effect on Tat activity in plasmid transfection assays. In contrast, alanine substitutions of all eight noncysteines analyzed, from residues 26 through 41, significantly reduced the activity of the Tat protein, in some cases as drastically as mutations in cysteine residues. The results demonstrate that the precise sequence of the cysteine-rich region is crucial for a fully functional Tat protein.

MeSH Terms
Amino Acid Sequence Animals Cell Line Cysteine Gene Products, tat/genetics HIV-1/genetics Humans Molecular Sequence Data Mutation Trans-Activators/genetics Transfection tat Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, tat Trans-Activators tat Gene Products, Human Immunodeficiency Virus Cysteine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rice A P
Cold Spring Harbor Laboratory, New York 11724.
Carlotti F
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44 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.4.1864-1868.1990
Published
1990-04-00
Pages
1864-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249332
Subset
IM
Grants
NIAID NIH HHS · 1PO1 AI27270-01 · United States
External Links
PubMed source DOI: 10.1128/JVI.64.4.1864-1868.1990 PMC Full Text View journal details
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