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PMID: 2181286 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Specific expression of human c-fgr in natural immunity effector cells.

Molecular and cellular biology ·Vol. 10 ·No. 4 ·1990-04-00 ·Pages 1789-92

Inoue K, Yamamoto T, Toyoshima K

Abstract

The c-fgr gene product was shown by immune complex protein kinase assay with specific antibodies to be a 58-kilodalton protein (p58c-fgr) with tyrosine-specific autophosphorylating activity. On examination of peripheral blood cells by immunoblotting with anti-c-fgr antibodies, p58c-fgr was found only in the fractions of monocytes, granulocytes, and natural killer cells. On the other hand, histochemical studies of hybridization demonstrated accumulation of c-fgr transcripts on most monocytes and large lymphocytes. In hematopoietic cell lines, p58c-fgr was detected in differentiated granulocytic cells as well as in differentiated monocytic cells of HL-60-cell origin. These data suggest a specific role for p58c-fgr in natural immunity effector cells.

MeSH Terms
Animals Antibodies Antigen-Antibody Complex/analysis Cells, Cultured Humans Immunity, Innate Killer Cells, Natural/enzymology,immunology Leukocytes/enzymology Molecular Weight Nucleic Acid Hybridization Phosphorylation Protein-Tyrosine Kinases/genetics Proto-Oncogene Proteins/genetics RNA, Messenger/genetics T-Lymphocytes/enzymology,immunology src-Family Kinases
Chemicals
Antibodies Antigen-Antibody Complex Proto-Oncogene Proteins RNA, Messenger Protein-Tyrosine Kinases proto-oncogene proteins c-fgr src-Family Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Inoue K
Department of Oncology, University of Tokyo, Japan.
Yamamoto T
Toyoshima K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.4.1789-1792.1990
Published
1990-04-00
Pages
1789-92
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362287
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.10.4.1789-1792.1990 PMC Full Text View journal details
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