Characterization of the genomic RNA from a Rous sarcoma virus mutant temperature sensitive for cell transformation.

Nucleic acids research ·Vol. 6 ·No. 2 ·1979-02-00 ·Pages 471-85

Darlix JL, Levray M, Bromley PA, Spahr PF

Abstract

We have found that the LA23 t/s mutant of Rous sarcoma virus (phenotype Prague B), even when passaged repeatedly at high multiplicity of infection, does not give rise to transformation defective deletion mutants comparable to those derived from RSV. In view of this fact and of the high rate of production of this mutant at 41 degrees C, we have undertaken a detailed analysis of the genome of this virus by ordering all large T1 oligonucleotides and by determining their nucleotide sequences. The results indicate a high degree of mutation in the onc gene as compared to that of Pr-A or Pr-B.

MeSH Terms

Avian Sarcoma Viruses/analysis Base Sequence Cell Transformation, Viral Genes, Viral Mutation Oligoribonucleotides/analysis RNA, Viral/analysis Ribonuclease T1 Temperature

Chemicals

Oligoribonucleotides RNA, Viral Ribonuclease T1

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Darlix J L

Levray M

Bromley P A

Spahr P F

References (15)

15 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.2.471

Published

1979-02-00

Pages

471-85

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327708

Subset

External Links

PubMed source DOI: 10.1093/nar/6.2.471 PMC Full Text View journal details

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Characterization of the genomic RNA from a Rous sarcoma virus mutant temperature sensitive for cell transformation.

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