Home LiteratureArticle Details
PMID: 218204 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Enkephalin inhibits release of substance P from sensory neurons in culture and decreases action potential duration.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 1 ·1979-01-00 ·Pages 526-30

Mudge AW, Leeman SE, Fischbach GD

Abstract

Sensory neurons grown in dispersed cell culture in the absence of non-neuronal cell types contain immunoreactive substance P that is chemically similar to synthetic substance P. When depolarized in high-K+ media (30-120 mM), the neurons release this peptide by a Ca2+-dependent mechanism. An enkephalin analogue, [D-Ala2]enkephalin amide, at 10 micron inhibits the K+-evoked release of substance P. At the same or lower concentrations, [D-Ala2]enkephalin amide and enkephalin decrease the duration of the Ca2+ action potential evoked and recorded in dorsal root ganglion cell bodies without affecting the resting membrane potential or resting membrane conductance. This modulation of voltage-sensitive channels may account for the inhibition of substance P release.

MeSH Terms
Action Potentials/drug effects Animals Cells, Cultured Chick Embryo Electric Conductivity Endorphins/pharmacology Enkephalins/pharmacology Ganglia, Spinal/drug effects,metabolism Membrane Potentials/drug effects Nerve Endings/drug effects Substance P/metabolism Synaptic Transmission/drug effects
Chemicals
Endorphins Enkephalins Substance P
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mudge A W
Leeman S E
Fischbach G D
References (27)
27 references, click to expand
  1. Graded and all-or-none electrogenesis in arthropod muscle. II. The effects of alkali-earth and onium ions on lobster muscle fibers.
    J Gen Physiol. 1961 May;44:997-1027 PMID: 13784437
  2. The action of morphine and related substances on contraction and on acetylcholine output of coaxially stimulated guinea-pig ileum.
    Br J Pharmacol Chemother. 1957 Mar;12(1):119-27 PMID: 13413163
  3. The action potential of chick dorsal root ganglion neurones maintained in cell culture.
    J Physiol. 1977 May;267(2):281-98 PMID: 559758
  4. Opioid peptide enkephalin: immunohistochemical mapping in rat central nervous system.
    Proc Natl Acad Sci U S A. 1977 May;74(5):2167-71 PMID: 194249
  5. Enkephalins and dorsal horn neurones of the cat: effects on responses to noxious and innocuous skin stimuli.
    Br J Pharmacol. 1977 Nov;61(3):399-408 PMID: 588805
  6. Regulation of noradrenaline release by presynaptic receptor systems.
    Rev Physiol Biochem Pharmacol. 1977;77:1-124 PMID: 14389
  7. Presynaptic modulation of voltage-dependent Ca2+ current: mechanism for behavioral sensitization in Aplysia californica.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3512-6 PMID: 28527
  8. Opiate analgesics inhibit substance P release from rat trigeminal nucleus.
    Nature. 1977 Aug 11;268(5620):549-51 PMID: 18681
  9. Release of substance P-like immunoreactivity from isolated spinal cord of newborn rat.
    Nature. 1976 Nov 4;264(5581):83-4 PMID: 12474
  10. Specific-opiate-induced depression of transmitter release from dorsal root ganglion cells in culture.
    Science. 1978 Mar 31;199(4336):1449-51 PMID: 204015
  11. The mechanism of action of narcotic analgesics in the guinea-pig ileum.
    Br J Pharmacol. 1977 Dec;61(4):541-9 PMID: 202356
  12. Endogenous opioid peptides: multiple agonists and receptors.
    Nature. 1977 Jun 9;267(5611):495-9 PMID: 195217
  13. Immunohistochemical analysis of peptide pathways possibly related to pain and analgesia: enkephalin and substance P.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):3081-5 PMID: 331326
  14. Effect of substance P in cat dorsal horn neurones activated by noxious stimuli.
    Brain Res. 1977 Jun 3;128(1):164-9 PMID: 871905
  15. Ultrastructural localization of substance P in neurons of rat spinal cord.
    Brain Res. 1977 Feb 25;122(3):534-40 PMID: 843902
  16. Immunohistochemical evidence for separate populations of somatostatin-containing and substance P-containing primary afferent neurons in the rat.
    Neuroscience. 1976;1(2):131-6 PMID: 1004708
  17. The mechanism of inhibition of neuronal activity by opiates in the spinal cord of cat.
    Brain Res. 1976 Oct 8;115(1):111-28 PMID: 974735
  18. The action of cobalt ions on neuromuscular transmission in the frog.
    J Physiol. 1973 Nov;234(3):597-612 PMID: 4357999
  19. Calcium influx in active Aplysia neurones detected by injected aequorin.
    Nat New Biol. 1973 Mar 28;242(117):113-5 PMID: 4145281
  20. Potassium activation in Helix aspersa neurones under voltage clamp: a component mediated by calcium influx.
    J Physiol. 1975 Jul;249(2):211-39 PMID: 1177091
  21. Synapse formation between dissociated nerve and muscle cells in low density cell cultures.
    Dev Biol. 1972 Jun;28(2):407-29 PMID: 4338026
  22. Substance P and excitatory transmitter of primary sensory neurons.
    Cold Spring Harb Symp Quant Biol. 1976;40:135-43 PMID: 7378
  23. Effect of morphine on adrenergic transmission in the mouse vas deferens. Assessment of agonist and antogonist potencies of narcotic analgesics.
    Br J Pharmacol. 1975 Mar;53(3):371-81 PMID: 236796
  24. Identification of the motoneuron-depolarizing peptide in bovine dorsal root as hypothalamic substance P.
    Brain Res. 1974 Jun 14;73(1):59-69 PMID: 4407840
  25. Opiate receptor binding in primate spinal cord: distribution and changes after dorsal root section.
    Brain Res. 1976 Aug 13;112(2):407-12 PMID: 821584
  26. Effects of substance P on functionally identified units in cat spinal cord.
    Brain Res. 1976 Sep 24;114(3):439-51 PMID: 953765
  27. The isolation of the mouse nerve growth factor protein in a high molecular weight form.
    Biochemistry. 1967 Jul;6(7):2202-9 PMID: 6049455
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.1.526
Published
1979-01-00
Pages
526-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC382975
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.1.526 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2